|Cat. No. 252 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 gallery
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 558 to 689 from mouse ClC3 (UniProt Id: P51791)|
Reacts with: rat (P51792), mouse (P51791).
Other species not tested yet.
|Specificity||Epitope present in both splice variants of ClC 3. May cross react to ClC 4 and 5, due to sequence homology.|
|Matching control protein/peptide||252-0P|
The ClC 3 chloride channel, also referred to as ClCN 3, belongs to the voltage-gated chloride channels and is ubiquitously expressed throughout the brain and other tissues. Two splice variants of the protein have been described. ClC 3 resides on intracellular trafficking vesicles including synaptic vesicles. It mediates the exchange of chloride ions against protons. In contrast to the translocation of glutamate into synaptic vesicles, the VGAT mediated transport of GABA requires ClC 3. It has been postulated that ClC 3 provides chloride ions as charge balance during the acidification of the vesicle lumen by proton pumping of the V-ATPase.