Cat. No.: 251 011
Amount: 100 µg
|Cat. No. 251 011||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
ICC: 1 : 500 (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)|
Epitop: AA 2 to 14 from mouse β-Actin (UniProt Id: P60710)
Reacts with: rat (P60711), mouse (P60710), zebrafish, human (P60709).
Other species not tested yet.
|Specificity||May cross-react to α- and γ-actin due to sequence homology.|
ICC: methanol or PFA fixation