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beta-Actin antibody - 251 005

beta-Actin is a major constituent of the cytoskeleton
Guinea pig polyclonal purified antibody
Cat. No.: 251 005
Amount: 50 µg
Price: $460.00
Cat. No. 251 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity Reacts with: rat (P60711), mouse (P60710).
Other species not tested yet.
Specificity May cross-react to α- and γ-actin due to sequence homology.
Remarks

ICC: Methanol fixation is recommended.

Data sheet 251_005.pdf
Cat. No.: 251 005
Amount: 50 µg
Price: $460.00
Background
The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.