Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 247 002

GAPDH antibody - 247 002

GAPDH is a key player in glycolysis
Rabbit polyclonal antiserum
Cat. No.: 247 002
Amount: 200 µl
Price: $355.00
Cat. No. 247 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Clarity: 1 : 100 (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Clarity is a sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. Clearing is carried out by SDS and Focusclear treatment. For additional experimental details, please refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 1 to 335 from human GAPDH (UniProt Id: P04406)
Reactivity Reacts with: human (P04406), rat (P04797), mouse (P16858), zebrafish.
Other species not tested yet.
Remarks

Well established as a loading control for Western-blotting.
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet 247_002.pdf

References for GAPDH - 247 002

Lysophosphatidic Acid Stimulates Mitogenic Activity and Signaling in Human Neuroblastoma Cells through a Crosstalk with Anaplastic Lymphoma Kinase.
Dedoni S, Olianas MC, Onali P
Biomolecules (2024) 146: . 247 002 WB; tested species: human
PHF2-mediated H3K9me balance orchestrates heterochromatin stability and neural progenitor proliferation.
Aguirre S, Pappa S, Serna-Pujol N, Padilla N, Iacobucci S, Nacht AS, Vicent GP, Jordan A, de la Cruz X, Martínez-Balbás MA
EMBO reports (2024) : . 247 002 WB; tested species: mouse
Oleoylethanolamide and Palmitoylethanolamide Enhance IFNβ-Induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells.
Camoglio C, Balla J, Fadda P, Dedoni S
Molecules (Basel, Switzerland) (2024) 297: . 247 002 WB; tested species: human
Loss of interleukin 1 signaling causes impairment of microglia- mediated synapse elimination and autistic-like behaviour in mice.
Borreca A, Mantovani C, Desiato G, Corradini I, Filipello F, Elia CA, D'Autilia F, Santamaria G, Garlanda C, Morini R, Pozzi D, et al.
Brain, behavior, and immunity (2024) 117: 493-509. 247 002 WB; tested species: mouse
Changes in glial cell activation and extracellular vesicles production precede the onset of disease symptoms in transgenic hSOD1G93A pigs.
Golia MT, Frigerio R, Pucci S, Sironi F, Margotta C, Pasetto L, Testori C, Berrone E, Ingravalle F, Chiari M, Gori A, et al.
Experimental neurology (2024) 374: 114716. 247 002 WB; tested species: pig
Microglial large extracellular vesicles propagate early synaptic dysfunction in Alzheimer's disease.
Gabrielli M, Prada I, Joshi P, Falcicchia C, D'Arrigo G, Rutigliano G, Battocchio E, Zenatelli R, Tozzi F, Radeghieri A, Arancio O, et al.
Brain : a journal of neurology (2022) : . 247 002 WB; tested species: mouse
The histone demethylase PHF8 regulates astrocyte differentiation and function.
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso-Mantis M, Verderio C, de la Cruz X, Martínez-Balbás MA
Development (Cambridge, England) (2021) 14812: . 247 002 WB; tested species: mouse
Nrg1 haploinsufficiency alters inhibitory cortical circuits.
Navarro-Gonzalez C, Carceller H, Benito Vicente M, Serra I, Navarrete M, Domínguez-Canterla Y, Rodríguez-Prieto Á, González-Manteiga A, Fazzari P
Neurobiology of disease (2021) 157: 105442. 247 002 WB; tested species: mouse
Astrocytes-derived extracellular vesicles in motion at the neuron surface: Involvement of the prion protein.
D'Arrigo G, Gabrielli M, Scaroni F, Swuec P, Amin L, Pegoraro A, Adinolfi E, Di Virgilio F, Cojoc D, Legname G, Verderio C, et al.
Journal of extracellular vesicles (2021) 109: e12114. 247 002 WB; tested species: rat
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring.
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, Peano C, et al.
Immunity (2021) 5411: 2611-2631.e8. 247 002 WB; tested species: mouse
Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.
Juergens L, Bieniussa L, Voelker J, Hagen R, Rak K
Histochemistry and cell biology (2020) 1546: 671-681. 247 002 WB; tested species: human
Antidepressants induce profibrotic responses via the lysophosphatidic acid receptor LPA1.
Olianas MC, Dedoni S, Onali P
European journal of pharmacology (2020) : 172963. 247 002 WB; tested species: human
Inhibition of TNF-α-induced neuronal apoptosis by antidepressants acting through the lysophosphatidic acid receptor LPA1.
Olianas MC, Dedoni S, Onali P
Apoptosis : an international journal on programmed cell death (2019) : . 247 002 WB; tested species: mouse
Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons.
Fossati M, Assendorp N, Gemin O, Colasse S, Dingli F, Arras G, Loew D, Charrier C
Neuron (2019) : . 247 002 WB; tested species: mouse
A dual-AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock-out mice.
Al-Moyed H, Cepeda AP, Jung S, Moser T, Kügler S, Reisinger E
EMBO molecular medicine (2019) 111: . 247 002 WB; tested species: mouse
Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure.
Lombardi M, Parolisi R, Scaroni F, Bonfanti E, Gualerzi A, Gabrielli M, Kerlero de Rosbo N, Uccelli A, Giussani P, Viani P, Garlanda C, et al.
Acta neuropathologica (2019) : . 247 002 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 247 002 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 247 002 WB; tested species: mouse
Overexpression of SNX7 reduces Aβ production by enhancing lysosomal degradation of APP.
Xu S, Zhang L, Brodin L
Biochemical and biophysical research communications (2018) 4951: 12-19. 247 002 WB; tested species: human
Altered Expression Profile of IgLON Family of Neural Cell Adhesion Molecules in the Dorsolateral Prefrontal Cortex of Schizophrenic Patients.
Karis K, Eskla KL, Kaare M, Täht K, Tuusov J, Visnapuu T, Innos J, Jayaram M, Timmusk T, Weickert CS, Väli M, et al.
Frontiers in molecular neuroscience (2018) 11: 8. 247 002 WB; tested species: human
Promoter-Specific Expression and Genomic Structure of IgLON Family Genes in Mouse.
Vanaveski T, Singh K, Narvik J, Eskla KL, Visnapuu T, Heinla I, Jayaram M, Innos J, Lilleväli K, Philips MA, Vasar E, et al.
Frontiers in neuroscience (2017) 11: 38. 247 002 WB; tested species: mouse
Muscarinic Acetylcholine Receptors Potentiate 5'-Adenosine Monophosphate-Activated Protein Kinase Stimulation and Glucose Uptake Triggered by Thapsigargin-Induced Store-Operated Ca2+ Entry in Human Neuroblastoma Cells.
Olianas MC, Dedoni S, Onali P
Neurochemical research (2017) : . 247 002 WB; tested species: human
The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cells lacking functional GABAB receptors.
Olianas MC, Dedoni S, Onali P
European journal of pharmacology (2017) 794: 135-146. 247 002 WB
Defective Angiogenesis and Intraretinal Bleeding in Mouse Models With Disrupted Inner Retinal Lamination.
Simmons AB, Merrill MM, Reed JC, Deans MR, Edwards MM, Fuerst PG
Investigative ophthalmology & visual science (2016) 574: 1563-77. 247 002 WB; tested species: mouse
Novel axon projection after stress and degeneration in the Dscam mutant retina.
Fernandes KA, Bloomsburg SJ, Miller CJ, Billingslea SA, Merrill MM, Burgess RW, Libby RT, Fuerst PG
Molecular and cellular neurosciences (2016) 71: 1-12. 247 002 WB
Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β.
Cymerman IA, Gozdz A, Urbanska M, Milek J, Dziembowska M, Jaworski J
PloS one (2015) 107: e0134018. 247 002 WB; tested species: mouse
REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 247 002 WB
Protective properties of lysozyme on β-amyloid pathology: implications for Alzheimer disease.
Helmfors L, Boman A, Civitelli L, Nath S, Sandin L, Janefjord C, McCann H, Zetterberg H, Blennow K, Halliday G, Brorsson AC, et al.
Neurobiology of disease (2015) 83: 122-33. 247 002 WB
Type I interferons up-regulate the expression and signalling of p75 NTR/TrkA receptor complex in differentiated human SH-SY5Y neuroblastoma cells.
Dedoni S, Olianas MC, Ingianni A, Onali P
Neuropharmacology (2014) 79: 321-34. 247 002 WB; tested species: human
Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development.
Osório C, Chacón PJ, Kisiswa L, White M, Wyatt S, Rodríguez-Tébar A, Davies AM
Development (Cambridge, England) (2013) 14023: 4751-62. 247 002 WB; tested species: mouse
Interactome of the amyloid precursor protein APP in brain reveals a protein network involved in synaptic vesicle turnover and a close association with Synaptotagmin-1.
Kohli BM, Pflieger D, Mueller LN, Carbonetti G, Aebersold R, Nitsch RM, Konietzko U
Journal of proteome research (2012) 118: 4075-90. 247 002 WB
Computerized EEG in the comparison of oxyprothepin and fluphenazine decanoat.
Misurec J, Náhunek K, Svestka J, Cesková E
Activitas nervosa superior (1979) 213: 140. 247 002 Clarity; tested species: human
Cat. No.: 247 002
Amount: 200 µl
Price: $355.00
Lysophosphatidic Acid Stimulates Mitogenic Activity and Signaling in Human Neuroblastoma Cells through a Crosstalk with Anaplastic Lymphoma Kinase.
Dedoni S, Olianas MC, Onali P
Biomolecules (2024) 146: . 247 002 WB; tested species: human
PHF2-mediated H3K9me balance orchestrates heterochromatin stability and neural progenitor proliferation.
Aguirre S, Pappa S, Serna-Pujol N, Padilla N, Iacobucci S, Nacht AS, Vicent GP, Jordan A, de la Cruz X, Martínez-Balbás MA
EMBO reports (2024) : . 247 002 WB; tested species: mouse
Oleoylethanolamide and Palmitoylethanolamide Enhance IFNβ-Induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells.
Camoglio C, Balla J, Fadda P, Dedoni S
Molecules (Basel, Switzerland) (2024) 297: . 247 002 WB; tested species: human
Loss of interleukin 1 signaling causes impairment of microglia- mediated synapse elimination and autistic-like behaviour in mice.
Borreca A, Mantovani C, Desiato G, Corradini I, Filipello F, Elia CA, D'Autilia F, Santamaria G, Garlanda C, Morini R, Pozzi D, et al.
Brain, behavior, and immunity (2024) 117: 493-509. 247 002 WB; tested species: mouse
Changes in glial cell activation and extracellular vesicles production precede the onset of disease symptoms in transgenic hSOD1G93A pigs.
Golia MT, Frigerio R, Pucci S, Sironi F, Margotta C, Pasetto L, Testori C, Berrone E, Ingravalle F, Chiari M, Gori A, et al.
Experimental neurology (2024) 374: 114716. 247 002 WB; tested species: pig
Microglial large extracellular vesicles propagate early synaptic dysfunction in Alzheimer's disease.
Gabrielli M, Prada I, Joshi P, Falcicchia C, D'Arrigo G, Rutigliano G, Battocchio E, Zenatelli R, Tozzi F, Radeghieri A, Arancio O, et al.
Brain : a journal of neurology (2022) : . 247 002 WB; tested species: mouse
The histone demethylase PHF8 regulates astrocyte differentiation and function.
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso-Mantis M, Verderio C, de la Cruz X, Martínez-Balbás MA
Development (Cambridge, England) (2021) 14812: . 247 002 WB; tested species: mouse
Nrg1 haploinsufficiency alters inhibitory cortical circuits.
Navarro-Gonzalez C, Carceller H, Benito Vicente M, Serra I, Navarrete M, Domínguez-Canterla Y, Rodríguez-Prieto Á, González-Manteiga A, Fazzari P
Neurobiology of disease (2021) 157: 105442. 247 002 WB; tested species: mouse
Astrocytes-derived extracellular vesicles in motion at the neuron surface: Involvement of the prion protein.
D'Arrigo G, Gabrielli M, Scaroni F, Swuec P, Amin L, Pegoraro A, Adinolfi E, Di Virgilio F, Cojoc D, Legname G, Verderio C, et al.
Journal of extracellular vesicles (2021) 109: e12114. 247 002 WB; tested species: rat
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring.
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, Peano C, et al.
Immunity (2021) 5411: 2611-2631.e8. 247 002 WB; tested species: mouse
Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.
Juergens L, Bieniussa L, Voelker J, Hagen R, Rak K
Histochemistry and cell biology (2020) 1546: 671-681. 247 002 WB; tested species: human
Antidepressants induce profibrotic responses via the lysophosphatidic acid receptor LPA1.
Olianas MC, Dedoni S, Onali P
European journal of pharmacology (2020) : 172963. 247 002 WB; tested species: human
Inhibition of TNF-α-induced neuronal apoptosis by antidepressants acting through the lysophosphatidic acid receptor LPA1.
Olianas MC, Dedoni S, Onali P
Apoptosis : an international journal on programmed cell death (2019) : . 247 002 WB; tested species: mouse
Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons.
Fossati M, Assendorp N, Gemin O, Colasse S, Dingli F, Arras G, Loew D, Charrier C
Neuron (2019) : . 247 002 WB; tested species: mouse
A dual-AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock-out mice.
Al-Moyed H, Cepeda AP, Jung S, Moser T, Kügler S, Reisinger E
EMBO molecular medicine (2019) 111: . 247 002 WB; tested species: mouse
Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure.
Lombardi M, Parolisi R, Scaroni F, Bonfanti E, Gualerzi A, Gabrielli M, Kerlero de Rosbo N, Uccelli A, Giussani P, Viani P, Garlanda C, et al.
Acta neuropathologica (2019) : . 247 002 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 247 002 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 247 002 WB; tested species: mouse
Overexpression of SNX7 reduces Aβ production by enhancing lysosomal degradation of APP.
Xu S, Zhang L, Brodin L
Biochemical and biophysical research communications (2018) 4951: 12-19. 247 002 WB; tested species: human
Altered Expression Profile of IgLON Family of Neural Cell Adhesion Molecules in the Dorsolateral Prefrontal Cortex of Schizophrenic Patients.
Karis K, Eskla KL, Kaare M, Täht K, Tuusov J, Visnapuu T, Innos J, Jayaram M, Timmusk T, Weickert CS, Väli M, et al.
Frontiers in molecular neuroscience (2018) 11: 8. 247 002 WB; tested species: human
Promoter-Specific Expression and Genomic Structure of IgLON Family Genes in Mouse.
Vanaveski T, Singh K, Narvik J, Eskla KL, Visnapuu T, Heinla I, Jayaram M, Innos J, Lilleväli K, Philips MA, Vasar E, et al.
Frontiers in neuroscience (2017) 11: 38. 247 002 WB; tested species: mouse
Muscarinic Acetylcholine Receptors Potentiate 5'-Adenosine Monophosphate-Activated Protein Kinase Stimulation and Glucose Uptake Triggered by Thapsigargin-Induced Store-Operated Ca2+ Entry in Human Neuroblastoma Cells.
Olianas MC, Dedoni S, Onali P
Neurochemical research (2017) : . 247 002 WB; tested species: human
The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cells lacking functional GABAB receptors.
Olianas MC, Dedoni S, Onali P
European journal of pharmacology (2017) 794: 135-146. 247 002 WB
Defective Angiogenesis and Intraretinal Bleeding in Mouse Models With Disrupted Inner Retinal Lamination.
Simmons AB, Merrill MM, Reed JC, Deans MR, Edwards MM, Fuerst PG
Investigative ophthalmology & visual science (2016) 574: 1563-77. 247 002 WB; tested species: mouse
Novel axon projection after stress and degeneration in the Dscam mutant retina.
Fernandes KA, Bloomsburg SJ, Miller CJ, Billingslea SA, Merrill MM, Burgess RW, Libby RT, Fuerst PG
Molecular and cellular neurosciences (2016) 71: 1-12. 247 002 WB
Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β.
Cymerman IA, Gozdz A, Urbanska M, Milek J, Dziembowska M, Jaworski J
PloS one (2015) 107: e0134018. 247 002 WB; tested species: mouse
REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 247 002 WB
Protective properties of lysozyme on β-amyloid pathology: implications for Alzheimer disease.
Helmfors L, Boman A, Civitelli L, Nath S, Sandin L, Janefjord C, McCann H, Zetterberg H, Blennow K, Halliday G, Brorsson AC, et al.
Neurobiology of disease (2015) 83: 122-33. 247 002 WB
Type I interferons up-regulate the expression and signalling of p75 NTR/TrkA receptor complex in differentiated human SH-SY5Y neuroblastoma cells.
Dedoni S, Olianas MC, Ingianni A, Onali P
Neuropharmacology (2014) 79: 321-34. 247 002 WB; tested species: human
Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development.
Osório C, Chacón PJ, Kisiswa L, White M, Wyatt S, Rodríguez-Tébar A, Davies AM
Development (Cambridge, England) (2013) 14023: 4751-62. 247 002 WB; tested species: mouse
Interactome of the amyloid precursor protein APP in brain reveals a protein network involved in synaptic vesicle turnover and a close association with Synaptotagmin-1.
Kohli BM, Pflieger D, Mueller LN, Carbonetti G, Aebersold R, Nitsch RM, Konietzko U
Journal of proteome research (2012) 118: 4075-90. 247 002 WB
Computerized EEG in the comparison of oxyprothepin and fluphenazine decanoat.
Misurec J, Náhunek K, Svestka J, Cesková E
Activitas nervosa superior (1979) 213: 140. 247 002 Clarity; tested species: human
WB
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), also referred to as G3PDH, catalyzes the sixth step of glycolysis and thus plays a crucial role in the glucose metabolism.
In addition to this well known metabolic function it also exhibits nitrosylase activities and is involved in cellular events like transcription, RNA transport, DNA replication and apoptosis. It is an abundant housekeeping gene and is frequently used as a loading reference in westernblot experiments.