Synaptic Systems

Cat. No.: 244 103

Amount: 50 µg

Price: $375.00

Cat. No. 244 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 42 to 60 from rat GluN2B (UniProt Id: Q00960)
Reactivity	Reacts with: rat (Q00960), mouse (Q01097). Other species not tested yet.
Specificity	Specific for GluN 2B, no cross-reactivity to GluN 2A.
Matching control protein/peptide	244-1P
Remarks	IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.
Data sheet	244_103.pdf

References for GluN2B - 244 103

Chronic mild corticosterone exposure during adolescence enhances behaviors and upregulates neuroplasticity-related proteins in rat hippocampus.
Li J, Li Y, Sun Y, Wang H, Liu X, Zhao Y, Wang H, Su Y, Si T
Progress in neuro-psychopharmacology & biological psychiatry (2019) 89: 400-411. 244 103 WB; tested species: rat

Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 244 103 WB; tested species: mouse

Hippocampal Memory Recovery After Acute Stress: A Behavioral, Morphological and Molecular Study.
Aguayo FI, Tejos-Bravo M, Díaz-Véliz G, Pacheco A, García-Rojo G, Corrales W, Olave FA, Aliaga E, Ulloa JL, Avalos AM, Román-Albasini L, et al.
Frontiers in molecular neuroscience (2018) 11: 283. 244 103 WB; tested species: rat

Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats.
Pacheco A, Aguayo FI, Aliaga E, Muñoz M, García-Rojo G, Olave FA, Parra-Fiedler NA, García-Pérez A, Tejos-Bravo M, Rojas PS, Parra CS, et al.
Frontiers in molecular neuroscience (2017) 10: 244. 244 103 WB, IHC; tested species: rat

Parental THC exposure leads to compulsive heroin-seeking and altered striatal synaptic plasticity in the subsequent generation.
Szutorisz H, DiNieri JA, Sweet E, Egervari G, Michaelides M, Carter JM, Ren Y, Miller ML, Blitzer RD, Hurd YL
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 396: 1315-23. 244 103 WB; tested species: rat

Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 103 WB

NWD1 facilitates synaptic transmission and contributes to neuropathic pain.
Wu Y, Fu Q, Huang X, Luo Y, Wan S, Peng M, Su S, Xu X, Li Y, Li X, Sun D, et al.
Neuropharmacology (2021) 205: 108919. 244 103 IHC; tested species: mouse

Cat. No.: 244 103

Amount: 50 µg

Price: $375.00

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Groc L et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Nagy GG et al., Eur. J. Neurosci. (2004)
3 ) Petralia RS et al., Eur. J. Neurosci. (2002)
4 ) Sans N et al., J. Neurosci. (2000)
5 ) Conti F et al., Cereb. Cortex (1999)
6 ) Petralia RS et al., J. Neurosci. (1994)
7 ) Monyer H et al., Science (1992)

Protocols

Standard protocol

OTHER

Preabsorbtion

Other products for this target

GluN2B antibody - 244 103

References for GluN2B - 244 103