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EEA 1 antibody - 237 002

EEA1 is an endosomal Rab 5 effector essential for endocytic membrane fusion
Polyclonal rabbit antiserum
Cat. No.: 237 002
Amount: 200 µl
Price: $350.00
Cat. No. 237 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 up to 1 : 2000 gallery  
IHC: not recommended
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Synthetic peptide corresponding to AA 2 to 13 from human EEA1 (UniProt Id: Q15075)
Reactivity Reacts with: human (Q15075), rat (A0A0G2K051), mouse (Q8BL66).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for EEA 1.
Data sheet 237_002.pdf

References for EEA 1 - 237 002

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 237 002 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 237 002 WB
Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 Family Member Calcyon.
Shi L, Hines T, Bergson C, Smith D
Molecular biology of the cell (2018) : mbcE18010007. 237 002 ICC; tested species: monkey
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 237 002 ICC
Sorting in early endosomes reveals connections to docking- and fusion-associated factors.
Barysch SV, Aggarwal S, Jahn R, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2009) 10624: 9697-702. 237 002
SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002
Cat. No.: 237 002
Quantity: 200 µl
Price: $350.00
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 237 002 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 237 002 WB
Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 Family Member Calcyon.
Shi L, Hines T, Bergson C, Smith D
Molecular biology of the cell (2018) : mbcE18010007. 237 002 ICC; tested species: monkey
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 237 002 ICC
Sorting in early endosomes reveals connections to docking- and fusion-associated factors.
Barysch SV, Aggarwal S, Jahn R, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2009) 10624: 9697-702. 237 002
SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002
Background
Extracellular compounds are internalized by endocytosis into so called endocytic vesicles. They fuse with early endosomes, from where the endocytosed material can be shuttled to a number of alternative destinations.
Early endosomal antigen 1 (EEA 1) is a peripheral membrane protein that locates to early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of Rab5.
Autoantibodies against EEA 1 have been shown to be associated with subacute cutaneous systemic lupus erythematosus.