Synaptic Systems

Cat. No.: 237 002

Amount: 200 µl

Price: $355.00

Cat. No. 237 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: not recommended IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 13 from human EEA1 (UniProt Id: Q15075)
Reactivity	Reacts with: human (Q15075), rat (A0A0G2K051), mouse (Q8BL66). No signal: zebrafish. Other species not tested yet.
Data sheet	237_002.pdf

References for EEA1 - 237 002

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 237 002 WB

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 237 002 WB

Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 Family Member Calcyon.
Shi L, Hines T, Bergson C, Smith D
Molecular biology of the cell (2018) : mbcE18010007. 237 002 ICC; tested species: monkey

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 237 002 ICC

Sorting in early endosomes reveals connections to docking- and fusion-associated factors.
Barysch SV, Aggarwal S, Jahn R, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2009) 10624: 9697-702. 237 002

SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002

Cat. No.: 237 002

Amount: 200 µl

Price: $355.00

SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002

ICC

IHC-P

Background

Extracellular compounds are internalized by endocytosis into so called endocytic vesicles. They fuse with early endosomes, from where the endocytosed material can be shuttled to a number of alternative destinations.
Early endosomal antigen 1 (EEA 1) is a peripheral membrane protein that locates to early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of Rab5.
Autoantibodies against EEA 1 have been shown to be associated with subacute cutaneous systemic lupus erythematosus.

1 ) Bergeland T et al., EMBO Rep. (2008)
2 ) Wilson JM et al., Mol. Biol. Cell (2000)
3 ) Simonsen A et al., J. Biol. Chem. (1999)
4 ) Callaghan J et al., Biochem. J. (1999)
5 ) Stenmark H et al., J. Biol. Chem. (1996)
6 ) Mu FT et al., J. Biol. Chem. (1995)

Protocols

ICC

IHC-P

Other products for this target

EEA1 antibody - 237 002

References for EEA1 - 237 002