|Cat. No. 234 017||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 1000 gallery
|Subtype||IgG2b (λ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 131 to 146 from mouse IBA1 (UniProt Id: Q9EQW9)|
Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
|Specificity||Specific for IBA 1|
Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).