Synaptic Systems

Cat. No.: 234 013

Amount: 50 µg

Price: $385.00

Cat. No. 234 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P: 1 : 1000 gallery
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity	Reacts with: rat (P55009), mouse (O70200), human (P55008). Other species not tested yet.
Specificity	K.O. validated
Data sheet	234_013.pdf

References for IBA1 - 234 013

ICC (3)
IHC (10)
IHC-P (1)

Evaluating CXCL12 for Effects on Reactive Gene Expression in Primary Astrocytes.
Zieger K, Cao C, Engele J
Journal of molecular neuroscience : MN (2024) 742: 57. 234 013 ICC; tested species: rat

Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Kempf J, Knelles K, Hersbach BA, Petrik D, Riedemann T, Bednarova V, Janjic A, Simon-Ebert T, Enard W, Smialowski P, Götz M, et al.
Cell reports (2021) 363: 109409. 234 013 ICC; tested species: mouse

A modified protocol for isolation of retinal microglia from the pig.
Zhang Y, Erhard AL, Plagemann T, Eter N, Heiduschka P
Experimental eye research (2021) 207: 108584. 234 013 ICC; tested species: pig

Redefining the ontogeny of hyalocytes as yolk sac-derived tissue-resident macrophages of the vitreous body.
Rosmus DD, Koch J, Hausmann A, Chiot A, Arnhold F, Masuda T, Kierdorf K, Hansen SM, Kuhrt H, Fröba J, Wolf J, et al.
Journal of neuroinflammation (2024) 211: 168. 234 013 IHC; tested species: mouse

Longitudinal imaging of vitreal hyperreflective foci in mice with acute optic nerve damage using visible-light optical coherence tomography.
Fan W, Miller DA, Chang S, Kweon J, Yeo WH, Grannonico M, Liu X, Zhang HF
Optics letters (2024) 498: 1880-1883. 234 013 IHC; tested species: mouse

Developmental profile of microglia distribution in nuclei of the superior olivary complex.
Zacher AC, Hohaus K, Felmy F, Pätz-Warncke C
The Journal of comparative neurology (2023) : . 234 013 IHC

Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny`s neocortex and hippocampus: Impact on synaptic markers.
Isaac AR, de Velasco PC, Fraga KYD, Tavares-do-Carmo MDG, Campos RMP, Iannotti FA, Verde R, Martins DBG, Santos TA, Ferreira BK, de Mello FG, et al.
The Journal of nutritional biochemistry (2021) 96: 108782. 234 013 IHC; tested species: rat

Classification of Microglial Morphological Phenotypes Using Machine Learning.
Leyh J, Paeschke S, Mages B, Michalski D, Nowicki M, Bechmann I, Winter K
Frontiers in cellular neuroscience (2021) 15: 701673. 234 013 IHC; tested species: mouse

Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice.
Murenu E, Kostidis S, Lahiri S, Geserich AS, Imhof A, Giera M, Michalakis S
International journal of molecular sciences (2021) 225: . 234 013 IHC; tested species: mouse

A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting.
Murenu E, Pavlou M, Richter L, Rapti K, Just S, Cehajic-Kapetanovic J, Tafrishi N, Hayes A, Scholey R, Lucas R, Büning H, et al.
Molecular therapy. Methods & clinical development (2021) 20: 587-600. 234 013 IHC; tested species: mouse

Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse

Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury.
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM
Cellular and molecular life sciences : CMLS (2020) : . 234 013 IHC; tested species: mouse

Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization.
Schlecht A, Zhang P, Wolf J, Thien A, Rosmus DD, Boneva S, Schlunck G, Lange C, Wieghofer P
Frontiers in cell and developmental biology (2020) 8: 618598. 234 013 IHC; tested species: human

Peptidoglycan accumulates in distinct brain regions and cell types over lifetime but is absent in newborns.
Zeiher C, Kuhrt H, Rifflet A, Winter K, Boon L, Stassart RM, Nutma E, Middeldorp J, Strating IM, Boneca IG, Bechmann I, et al.
Brain, behavior, and immunity (2024) 123: 799-812. 234 013 IHC-P; tested species: human

Cat. No.: 234 013

Amount: 50 µg

Price: $385.00

References
ICC (3)
IHC (10)
IHC-P (1)

Evaluating CXCL12 for Effects on Reactive Gene Expression in Primary Astrocytes.
Zieger K, Cao C, Engele J
Journal of molecular neuroscience : MN (2024) 742: 57. 234 013 ICC; tested species: rat

Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).

1 ) Köhler C et al., Cell Tissue Res. (2007)
2 ) Deininger MH et al., Acta Neuropathol. (2000)
3 ) Gehrmann J et al., Brain Res. Brain Res. Rev. (1995)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

IBA1 antibody - 234 013 K.O.

References for IBA1 - 234 013