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IBA1 antibody - 234 013

IBA1 is a marker for macrophages and microglia
Polyclonal rabbit purified antibody
Cat. No.: 234 013
Amount: 50 µg
Price: $370.00
Cat. No. 234 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000      gallery  
IHC-P/FFPE: 1 : 1000      gallery  
Immunogen Synthetic peptide corresponding to AA 131 to 146 from mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: rat (P55009), mouse (O70200), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1. K.O.
Data sheet 234_013.pdf

References for IBA1 - 234 013

Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Kempf J, Knelles K, Hersbach BA, Petrik D, Riedemann T, Bednarova V, Janjic A, Simon-Ebert T, Enard W, Smialowski P, Götz M, et al.
Cell reports (2021) 363: 109409. 234 013 ICC; tested species: mouse
Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse
Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury.
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM
Cellular and molecular life sciences : CMLS (2020) : . 234 013 IHC; tested species: mouse
Cat. No.: 234 013
Quantity: 50 µg
Price: $370.00
Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Kempf J, Knelles K, Hersbach BA, Petrik D, Riedemann T, Bednarova V, Janjic A, Simon-Ebert T, Enard W, Smialowski P, Götz M, et al.
Cell reports (2021) 363: 109409. 234 013 ICC; tested species: mouse
Undisturbed climbing fiber pruning in the cerebellar cortex of CX3 CR1-deficient mice.
Kaiser N, Pätz C, Brachtendorf S, Eilers J, Bechmann I
Glia (2020) : . 234 013 IHC; tested species: mouse
Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury.
Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM
Cellular and molecular life sciences : CMLS (2020) : . 234 013 IHC; tested species: mouse
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).