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Home > Products > 224 705

GABA-A receptor beta1 antibody - 224 705

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig polyclonal purified antibody
Cat. No.: 224 705
Amount: 50 µg
Price: $460.00
Cat. No. 224 705 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes
Immunogen Recombinant protein corresponding to AA 342 to 430 from rat GABA-A receptor β1 (UniProt Id: P15431)
Reactivity Reacts with: rat (P15431), mouse (P50571).
Other species not tested yet.
Remarks

WB:  GABA-A receptor beta1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
Data sheet 224_705.pdf

References for GABA-A receptor beta1 - 224 705

Prepubescent female rodents have enhanced hippocampal LTP and learning relative to males, reversing in adulthood as inhibition increases.
Le AA, Lauterborn JC, Jia Y, Wang W, Cox CD, Gall CM, Lynch G
Nature neuroscience (2022) 252: 180-190. 224 705 IHC; tested species: rat
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 705 IHC; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 705 EM
Cat. No.: 224 705
Amount: 50 µg
Price: $460.00
Prepubescent female rodents have enhanced hippocampal LTP and learning relative to males, reversing in adulthood as inhibition increases.
Le AA, Lauterborn JC, Jia Y, Wang W, Cox CD, Gall CM, Lynch G
Nature neuroscience (2022) 252: 180-190. 224 705 IHC; tested species: rat
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 705 IHC; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 705 EM
WB
ICC
IHC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.
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