Synaptic Systems - GABA-A receptor alpha5

Cat. No.: 224 503

Amount: 50 µg

Price: $460.00

Cat. No. 224 503	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 26 to 43 from rat GABA-A receptor α5 (UniProt Id: P19969)
Reactivity	Reacts with: rat (P19969), mouse (Q8BHJ7). Other species not tested yet.
Specificity	K.O. validated PubMed: 27792253
Matching control protein/peptide	224-5P
Remarks	WB: GABA-A receptor alpha5 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	224_503.pdf

References for GABA-A receptor alpha5 - 224 503

Inhibitory and excitatory synaptic neuroadaptations in the diazepam tolerant brain.
Lorenz-Guertin JM, Povysheva N, Chapman CA, MacDonald ML, Fazzari M, Nigam A, Nuwer JL, Das S, Brady ML, Vajn K, Bambino MJ, et al.
Neurobiology of disease (2023) : 106248. 224 503 WB; tested species: mouse

Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticity.
Wu K, Han W, Lu W
PLoS biology (2022) 2011: e3001812. 224 503 WB; tested species: mouse

Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.
Wu K, Castellano D, Tian Q, Lu W
Cell reports (2021) 376: 109960. 224 503 WB, UPTAKE; tested species: mouse

Sustained treatment with an α5 GABA A receptor negative allosteric modulator delays excitatory circuit development while maintaining GABAergic neurotransmission.
Nuwer JL, Brady ML, Povysheva NV, Coyne A, Jacob TC
Neuropharmacology (2021) 197: 108724. 224 503 WB, ICC; tested species: rat

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse

Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress.
Everington EA, Gibbard AG, Swinny JD, Seifi M
Frontiers in molecular neuroscience (2018) 11: 18. 224 503 WB; tested species: mouse

Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 503 WB

Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 224 503 WB, IP, ICC; tested species: rat

FMRP regulates GABAA receptor channel activity to control signal integration in hippocampal granule cells.
Deng PY, Kumar A, Cavalli V, Klyachko VA
Cell reports (2022) 397: 110820. 224 503 IP; tested species: mouse

Long-term α5 GABA A receptor negative allosteric modulator treatment reduces NMDAR-mediated neuronal excitation and maintains basal neuronal inhibition.
Nuwer JL, Povysheva N, Jacob TC
Neuropharmacology (2023) 237: 109587. 224 503 ICC; tested species: rat

Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis.
Ichinose S, Susuki Y, Hosoi N, Kaneko R, Ebihara M, Hirai H, Iwasaki H
eLife (2023) 12: . 224 503 ICC; tested species: mouse

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse

Alternative Splicing and the Intracellular Domain Mediate TM-agrin's Ability to Differentially Regulate the Density of Excitatory and Inhibitory Synapse-like Specializations in Developing CNS Neurons.
Handara G, Kröger S
Neuroscience (2019) : . 224 503 ICC; tested species: mouse

Sparsification of AP firing in adult-born hippocampal granule cells via voltage-dependent α5-GABAA receptors.
Lodge M, Hernandez MC, Schulz JM, Bischofberger J
Cell reports (2021) 371: 109768. 224 503 IHC; tested species: mouse

Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 503 IHC; tested species: rat

Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 503 IHC; tested species: mouse

α5GABAA Receptors Mediate Tonic Inhibition in the Spinal Cord Dorsal Horn and Contribute to the Resolution Of Hyperalgesia.
Perez-Sanchez J, Lorenzo LE, Lecker I, Zurek AA, Labrakakis C, Bridgwater EM, Orser BA, De Koninck Y, Bonin RP
Journal of neuroscience research (2017) 956: 1307-1318. 224 503 IHC; KO verified; tested species: mouse

Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.
Wu K, Castellano D, Tian Q, Lu W
Cell reports (2021) 376: 109960. 224 503 WB, UPTAKE; tested species: mouse

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse

Cat. No.: 224 503

Amount: 50 µg

Price: $460.00

Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticity.
Wu K, Han W, Lu W
PLoS biology (2022) 2011: e3001812. 224 503 WB; tested species: mouse

Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.
Wu K, Castellano D, Tian Q, Lu W
Cell reports (2021) 376: 109960. 224 503 WB, UPTAKE; tested species: mouse

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse

Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.
Wu K, Castellano D, Tian Q, Lu W
Cell reports (2021) 376: 109960. 224 503 WB, UPTAKE; tested species: mouse

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Laurie DJ et al., J. Neurosci. (1992)
2 ) Laurie DJ et al., J. Neurosci. (1992)
3 ) Wisden W et al., J. Neurosci. (1992)
4 ) Lüddens H et al., Nature (1990)
5 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

GABA-A receptor alpha5 antibody extracellular - 224 503 K.O.

References for GABA-A receptor alpha5 - 224 503