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Home > Products > 224 503

GABA-A receptor alpha5 antibody extracellular - 224 503 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 503
Amount: 50 µg
Price: $465.00
Cat. No. 224 503 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 26 to 43 from rat GABA-A receptor α5 (UniProt Id: P19969)
Reactivity Reacts with: rat (P19969), mouse (Q8BHJ7).
Other species not tested yet.
Specificity K.O. validated PubMed: 27792253
Matching control protein/peptide 224-5P
Remarks

WB:  GABA-A receptor alpha5 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 224_503.pdf

References for GABA-A receptor alpha5 - 224 503

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 503 WB, ICC; tested species: mouse
Inhibitory and excitatory synaptic neuroadaptations in the diazepam tolerant brain.
Lorenz-Guertin JM, Povysheva N, Chapman CA, MacDonald ML, Fazzari M, Nigam A, Nuwer JL, Das S, Brady ML, Vajn K, Bambino MJ, et al.
Neurobiology of disease (2023) : 106248. 224 503 WB; tested species: mouse
Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticity.
Wu K, Han W, Lu W
PLoS biology (2022) 2011: e3001812. 224 503 WB; tested species: mouse
Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.
Wu K, Castellano D, Tian Q, Lu W
Cell reports (2021) 376: 109960. 224 503 WB, UPTAKE; tested species: mouse
Sustained treatment with an α5 GABA A receptor negative allosteric modulator delays excitatory circuit development while maintaining GABAergic neurotransmission.
Nuwer JL, Brady ML, Povysheva NV, Coyne A, Jacob TC
Neuropharmacology (2021) 197: 108724. 224 503 WB, ICC; tested species: rat
Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.
Wu K, Han W, Tian Q, Li Y, Lu W
Cell reports (2021) 3412: 108899. 224 503 WB, ICC, UPTAKE; tested species: mouse
Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress.
Everington EA, Gibbard AG, Swinny JD, Seifi M
Frontiers in molecular neuroscience (2018) 11: 18. 224 503 WB; tested species: mouse
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 224 503 WB
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 224 503 WB, IP, ICC; tested species: rat
Cat. No.: 224 503
Amount: 50 µg
Price: $465.00
WB
ICC
IHC
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