|Cat. No. 224 503||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 26 to 43 from rat GABA-A receptor α5 (UniProt Id: P19969)|
Reacts with: rat (P19969), mouse (Q8BHJ7).
Other species not tested yet.
|Specificity||Specific for GABA-A receptor α5. K.O. PubMed: 27792253|
|Matching control protein/peptide||224-5P|
WB: GABA-A receptor alpha5 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.