GABA-A receptor beta3 antibody - 224 411BT

GABA-A receptors are involved in inhibitory neurotransmission

Mouse monoclonal purified IgG

Cat. No.: 224 411BT

Amount: 100 µg

Price: $465.00

Cat. No. 224 411BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) IP: yes ICC: 1 : 500 gallery IHC: not tested yet (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	261F6
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity	Reacts with: rat (P63079), mouse (P63080). Other species not tested yet.
Remarks	WB: Less sensitive compared to the polyclonal antibodies (224 403 and 224 404). IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	224_411bt.pdf

Cat. No.: 224 411BT

Amount: 100 µg

Price: $465.00

ICC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
2 ) Viltono L et al., J. Comp. Neurol. (2008)
3 ) Kralic JE et al., J. Comp. Neurol. (2006)
4 ) Essrich C et al., Nat. Neurosci. (1998)
5 ) Fritschy JM et al., J. Comp. Neurol. (1995)
6 ) Benke D et al., J. Biol. Chem. (1994)
7 ) Fritschy JM et al., Proc. Natl. Acad. Sci. U.S.A. (1992)

Protocols

Standard protocol

ICC

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