Synaptic Systems - GABA-A receptor beta3

Cat. No.: 224 411

Amount: 100 µg

Price: $420.00

Cat. No. 224 411	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	261F6
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity	Reacts with: rat (P63079), mouse (P63080). Other species not tested yet.
Remarks	WB: Less sensitive compared to the polyclonal antibodies (224 403 and 224 404). IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	224_411.pdf

References for GABA-A receptor beta3 - 224 411

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 411 WB, ICC; tested species: mouse

The Autism-Related Protein PX-RICS Mediates GABAergic Synaptic Plasticity in Hippocampal Neurons and Emotional Learning in Mice.
Nakamura T, Sakaue F, Nasu-Nishimura Y, Takeda Y, Matsuura K, Akiyama T
EBioMedicine (2018) : . 224 411 WB; tested species: mouse

Cat. No.: 224 411

Amount: 100 µg

Price: $420.00

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
2 ) Viltono L et al., J. Comp. Neurol. (2008)
3 ) Kralic JE et al., J. Comp. Neurol. (2006)
4 ) Essrich C et al., Nat. Neurosci. (1998)
5 ) Fritschy JM et al., J. Comp. Neurol. (1995)
6 ) Benke D et al., J. Biol. Chem. (1994)
7 ) Fritschy JM et al., Proc. Natl. Acad. Sci. U.S.A. (1992)

Protocols

Standard protocol

ICC

IHC

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GABA-A receptor beta3 antibody - 224 411

References for GABA-A receptor beta3 - 224 411