Tailor-made Antibodies
and Tools for Life Science

GABA-A receptor beta3 antibody - 224 403

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 403
Amount: 50 µg
Price: $380.00
Cat. No. 224 403 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity Reacts with: rat (P63079), mouse (P63080).
Other species not tested yet.
Remarks

WB:  GABA-A receptor beta3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended.
IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are recommended.

Data sheet 224_403.pdf

References for GABA-A receptor beta3 - 224 403

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 403 WB, ICC; tested species: mouse
Cat. No.: 224 403
Amount: 50 µg
Price: $380.00