GABA-A receptor alpha3 antibody extracellular - 224 302

GABA-A receptors are involved in inhibitory neurotransmission

Rabbit polyclonal antiserum

Cat. No.: 224 302

Amount: 200 µl

Price: $355.00

Cat. No. 224 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 43 from rat GABA-A receptor α3 (UniProt Id: P20236)
Reactivity	Reacts with: rat (P20236), mouse (P26049). Other species not tested yet.
Matching control protein/peptide	224-3P
Remarks	WB: GABA-A receptor alpha3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	224_302.pdf

References for GABA-A receptor alpha3 - 224 302

Wheel-Running Exercise Alleviates Anxiety-Like Behavior via Down-Regulating S-Nitrosylation of Gephyrin in the Basolateral Amygdala of Male Rats.
Yang PF, Nie TL, Sun XN, Xu LX, Ma C, Wang F, Long LH, Chen JG
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400205. 224 302 WB; tested species: rat

Cat. No.: 224 302

Amount: 200 µl

Price: $355.00

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

OTHER

Preabsorbtion

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