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GABA-A receptor alpha3 - 224 302

Receptors mediating inhibitory neurotransmission
Polyclonal rabbit antiserum
Cat. No.: 224 302
Amount: 200 µl
Price: 350.00 €
Cat. No. 224 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet (see remarks)
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 29 to 43 from rat GABA-A receptor α3 (UniProt Id: P20236)
Reactivity Reacts with: rat (P20236), mouse (P26049).
Other species not tested yet.
Specificity Specific for GABA-A receptor α3.
Matching control protein/peptide 224-3P
Remarks

WB:  GABA-A receptor alpha3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
 

Data sheet 224_302.pdf
Cat. No.: 224 302
Quantity: 200 µl
Price: 350.00 €
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.