Synaptic Systems - GABA-A receptor alpha2

Cat. No.: 224 104

Amount: 100 µl

Price: $370.00

Cat. No. 224 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity	Reacts with: human (P47869), rat (P23576), mouse (P26048). Other species not tested yet.
Matching control protein/peptide	224-1P
Remarks	WB: GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
Data sheet	224_104.pdf

References for GABA-A receptor alpha2 - 224 104

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 104 ICC; tested species: mouse

γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 104 ICC

Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 104 ICC; tested species: mouse

Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection.
Zhou W, He Y, Rehman AU, Kong Y, Hong S, Ding G, Yalamanchili HK, Wan YW, Paul B, Wang C, Gong Y, et al.
Nature neuroscience (2019) : . 224 104 IHC; tested species: mouse

Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.
Wefelmeyer W, Cattaert D, Burrone J
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: 9757-62. 224 104 IHC

Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 224 104 UPTAKE; tested species: mouse

Cat. No.: 224 104

Amount: 100 µl

Price: $370.00

ICC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

ICC

OTHER

Preabsorbtion

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GABA-A receptor alpha2 antibody extracellular - 224 104

References for GABA-A receptor alpha2 - 224 104