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GABA-A receptor gamma2 - 224 004

Receptors mediating inhibitory neurotransmission
Polyclonal Guinea pig antiserum
Cat. No.: 224 004
Amount: 100 µl
Price: 350.00 €
Cat. No. 224 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: yes limited (see remarks)
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity Reacts with: human (P18507), rat (P18508), mouse (P22723).
Other species not tested yet.
Specificity Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.
Matching control protein/peptide 224-0P
Remarks

WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Data sheet 224_004.pdf

References for GABA-A receptor gamma2 - 224 004

Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 WB, ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 004 ICC; tested species: rat
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 224 004 ICC; tested species: mouse
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Cell surface expression of homomeric GABAA receptors depends on single residues in subunit transmembrane domains.
Hannan S, Smart TG
The Journal of biological chemistry (2018) : . 224 004 ICC; tested species: human
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 WB, ICC
Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2012) 503-4: 250-9. 224 004 ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state.
Fuchs T, Jefferson SJ, Hooper A, Yee PH, Maguire J, Luscher B
Molecular psychiatry (2017) 226: 920-930. 224 004 IHC
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 004 IHC
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 224 004 IHC; tested species: mouse
SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 224 004 UPTAKE; tested species: rat
Cat. No.: 224 004
Quantity: 100 µl
Price: 350.00 €
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 WB, ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 004 ICC; tested species: rat
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 224 004 ICC; tested species: mouse
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Cell surface expression of homomeric GABAA receptors depends on single residues in subunit transmembrane domains.
Hannan S, Smart TG
The Journal of biological chemistry (2018) : . 224 004 ICC; tested species: human
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 WB, ICC
Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2012) 503-4: 250-9. 224 004 ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state.
Fuchs T, Jefferson SJ, Hooper A, Yee PH, Maguire J, Luscher B
Molecular psychiatry (2017) 226: 920-930. 224 004 IHC
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 004 IHC
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 224 004 IHC; tested species: mouse
SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 224 004 UPTAKE; tested species: rat
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.