Synaptic Systems - GABA-A receptor gamma2

Cat. No.: 224 004

Amount: 100 µl

Price: $375.00

Cat. No. 224 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes limited (see remarks) IP: yes ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery
Immunogen	Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity	Reacts with: human (P18507), rat (P18508), mouse (P22723). Other species not tested yet.
Specificity	Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.
Matching control protein/peptide	224-0P
Remarks	WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: For best results use the protocol of Schneider Gasser et al., 2006. Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining. IHC-Fr: PFA fixation is recommended.
Data sheet	224_004.pdf

Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model.
Kiss E, Kins S, Zöller Y, Schilling S, Gorgas K, Groß D, Schlicksupp A, Rosner R, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2021) 113: 103624. 224 004 WB, IHC; tested species: mouse

Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 ICC, WB