|Cat. No. 224 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: yes limited (see remarks)
ICC: 1 : 500 (see remarks) gallery
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)|
Reacts with: human (P18507), rat (P18508), mouse (P22723).
Other species not tested yet.
|Specificity||Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.|
|Matching control protein/peptide||224-0P|
WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.