Tailor-made Antibodies
and Tools for Life Science
my list | quick order | cart
0
|
login
Home > Products > 224 003

GABA-A receptor gamma2 antibody extracellular - 224 003 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 003
Amount: 50 µg
Price: $405.00
Cat. No. 224 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 (see remarks) gallery  
IHC-P: not tested yet
EM: yes
Immunogen Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity Reacts with: human (P18507), rat (P18508), mouse (P22723), monkey, zebrafish.
Other species not tested yet.
Specificity Specific for GABA-A receptor γ2. Does not discriminate between the L and S form. K.O. validated PubMed: 24849349
Matching control protein/peptide 224-0P
Remarks

WB:  GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Glyoxal fixation according to Konno et al. 2023 has improved signal quality and strength.
Data sheet 224_003.pdf

References for GABA-A receptor gamma2 - 224 003

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 003 WB, ICC; tested species: mouse
Activity in the dorsal hippocampus-mPFC circuit modulates stress-coping strategies during inescapable stress.
Yoon SH, Song WS, Chung G, Kim SJ, Kim MH
Experimental & molecular medicine (2024) : . 224 003 WB; tested species: mouse
Photoproximity labeling of endogenous receptors in the live mouse brain in minutes.
Takato M, Sakamoto S, Nonaka H, Tanimura Valor FY, Tamura T, Hamachi I
Nature chemical biology (2024) : . 224 003 WB; tested species: mouse
Wheel-Running Exercise Alleviates Anxiety-Like Behavior via Down-Regulating S-Nitrosylation of Gephyrin in the Basolateral Amygdala of Male Rats.
Yang PF, Nie TL, Sun XN, Xu LX, Ma C, Wang F, Long LH, Chen JG
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400205. 224 003 WB; tested species: rat
Inhibitory and excitatory synaptic neuroadaptations in the diazepam tolerant brain.
Lorenz-Guertin JM, Povysheva N, Chapman CA, MacDonald ML, Fazzari M, Nigam A, Nuwer JL, Das S, Brady ML, Vajn K, Bambino MJ, et al.
Neurobiology of disease (2023) : 106248. 224 003 WB, IP; tested species: mouse
Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 224 003 WB; tested species: mouse
Kv7/KCNQ potassium channels in cortical hyperexcitability and juvenile seizure-related death in Ank2-mutant mice.
Oh H, Lee S, Oh Y, Kim S, Kim YS, Yang Y, Choi W, Yoo YE, Cho H, Lee S, Yang E, et al.
Nature communications (2023) 141: 3547. 224 003 WB; tested species: mouse
Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 224 003 WB, ICC; tested species: rat
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 003 WB, UPTAKE; tested species: rat
REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.
Prestigio C, Ferrante D, Marte A, Romei A, Lignani G, Onofri F, Valente P, Benfenati F, Baldelli P
eLife (2021) 10: . 224 003 WB; tested species: mouse
Cerebellum-Specific Deletion of the GABAA Receptor δ Subunit Leads to Sex-Specific Disruption of Behavior.
Rudolph S, Guo C, Pashkovski SL, Osorno T, Gillis WF, Krauss JM, Nyitrai H, Flaquer I, El-Rifai M, Datta SR, Regehr WG, et al.
Cell reports (2020) 335: 108338. 224 003 WB; tested species: mouse
Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 224 003 WB, ICC; tested species: mouse
Calsyntenin-3 interacts with both α- and β-neurexins in the regulation of excitatory synaptic innervation in specific Schaffer collateral pathways.
Kim H, Kim D, Kim J, Lee HY, Park D, Kang H, Matsuda K, Sterky FH, Yuzaki M, Kim JY, Choi SY, et al.
The Journal of biological chemistry (2020) : . 224 003 WB, IHC; tested species: mouse
Aberrant expression of S-SCAM causes the loss of GABAergic synapses in hippocampal neurons.
Shin SM, Skaar S, Danielson E, Lee SH
Scientific reports (2020) 101: 83. 224 003 WB, ICC; tested species: rat
The small GTPase ARF6 regulates GABAergic synapse development.
Kim H, Jung H, Jung H, Kwon SK, Ko J, Um JW
Molecular brain (2020) 131: 2. 224 003 WB, ICC, IHC; tested species: rat
Diazepam Accelerates GABAAR Synaptic Exchange and Alters Intracellular Trafficking.
Lorenz-Guertin JM, Bambino MJ, Das S, Weintraub ST, Jacob TC
Frontiers in cellular neuroscience (2019) 13: 163. 224 003 WB, IP, ICC; tested species: rat
LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors.
Fu YL, Zhang B, Mu TW
Biochemical and biophysical research communications (2019) : . 224 003 WB; tested species: mouse
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 224 003 WB, ICC; tested species: rat
Activating Transcription Factor 4 (ATF4) regulates neuronal activity by controlling GABABR trafficking.
Corona C, Pasini S, Liu J, Amar F, Greene LA, Shelanski ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 224 003 WB; tested species: rat
GABAAand GABABreceptor subunit localization on neurochemically identified neurons of the human subthalamic nucleus.
Wu XH, Song JJ, Faull RLM, Waldvogel HJ
The Journal of comparative neurology (2018) 5265: 803-823. 224 003 WB, IHC; tested species: human
Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress.
Everington EA, Gibbard AG, Swinny JD, Seifi M
Frontiers in molecular neuroscience (2018) 11: 18. 224 003 WB; tested species: mouse
IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 224 003 WB
γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 003 WB
Cat. No.: 224 003
Amount: 50 µg
Price: $405.00
WB
ICC
IHC
Other products for this target