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GARP - 221 011

An orphan receptor on Treg-cells
Monoclonal mouse purified IgG
Cat. No.: 221 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 221 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone 50G10
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 23 to 612 from human GARP (UniProt Id: Q14392)
Epitop Epitop: AA 23 to 612 from human GARP (UniProt Id: Q14392)
Reactivity Reacts with: human (Q14392).
Other species not tested yet.
Specificity Specific for GARP.
Remarks Skimmed milk is required for efficient blocking.
Data sheet 221_011.pdf

References for GARP - 221 011

GARP: a key receptor controlling FOXP3 in human regulatory T cells.
Probst-Kepper M, Geffers R, Kröger A, Viegas N, Erck C, Hecht HJ, Lünsdorf H, Roubin R, Moharregh-Khiabani D, Wagner K, Ocklenburg F, et al.
Journal of cellular and molecular medicine (2009) 139B: 3343-57. 221 011 WB
Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo.
Cuende J, Liénart S, Dedobbeleer O, van der Woning B, De Boeck G, Stockis J, Huygens C, Colau D, Somja J, Delvenne P, Hannon M, et al.
Science translational medicine (2015) 7284: 284ra56. 221 011
Cat. No.: 221 011
Quantity: 100 µg
Price: 415.00 €
GARP: a key receptor controlling FOXP3 in human regulatory T cells.
Probst-Kepper M, Geffers R, Kröger A, Viegas N, Erck C, Hecht HJ, Lünsdorf H, Roubin R, Moharregh-Khiabani D, Wagner K, Ocklenburg F, et al.
Journal of cellular and molecular medicine (2009) 139B: 3343-57. 221 011 WB
Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo.
Cuende J, Liénart S, Dedobbeleer O, van der Woning B, De Boeck G, Stockis J, Huygens C, Colau D, Somja J, Delvenne P, Hannon M, et al.
Science translational medicine (2015) 7284: 284ra56. 221 011
Background
Regulatory T-cells (Treg-cells) are involved in the homeostasis of immune reactions and tolerance. These specialized T-cells suppress auto-reactive cells and inhibit autoimmunity. Development and function of Treg-cells depend on the expression of the transcription factor FOX3P.
The orphan receptor GARP, also referred to as LRRC 32, is exclusively found on Treg-cells upon stimulation with anti-CD3/IL-2 or anti-CD3/anti-CD28/IL-2. Additionally it has been shown, that ectopic GARP expression strongly induces FOXP3.