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Abeta 43 - 218 903

A major component of neuritic plaques in Alzheimer's disease
Polyclonal rabbit purified antibody
Cat. No.: 218 903
Amount: 50 µg
Price: $370.00
Cat. No. 218 903 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 100 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 38 to 43 from human Abeta43 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 43
Remarks

WB: Detects purified Abeta 43. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min.
IHC: Antigen retrieval with formic acid is required.
IHC-P: Antigen retrieval with formic acid is required.

Data sheet 218_903.pdf

References for Abeta 43 - 218 903

Immunohistochemical analysis of spinal cord components in mouse model of experimental autoimmune encephalomyelitis.
Pyka-Fosciak G, Stasiolek M, Litwin JA
Folia histochemica et cytobiologica (2018) 563: 151-158. 218 903 IHC; tested species: mouse
Cat. No.: 218 903
Quantity: 50 µg
Price: $370.00
Immunohistochemical analysis of spinal cord components in mouse model of experimental autoimmune encephalomyelitis.
Pyka-Fosciak G, Stasiolek M, Litwin JA
Folia histochemica et cytobiologica (2018) 563: 151-158. 218 903 IHC; tested species: mouse
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.