Tailor-made Antibodies
and Tools for Life Science

ChT antibody - 216 008

ChT is a high affinity choline transporter
Rabbit monoclonal recombinant IgG
Cat. No.: 216 008
Amount: 50 µg
Price: $420.00
Cat. No. 216 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 (see remarks) gallery  
Clone Rb62-2E8
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to residues near the C-terminus of rat ChT (UniProt Id: Q9JMD7)
Reactivity Reacts with: mouse (Q8BGY9), rat (Q9JMD7), monkey.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 62-2E8. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. This antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: ChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-P: Not recommended for rat tissue. Unspecific nuclear signal has been observed using our standard IHC-P protocol.

Data sheet 216_008.pdf
Cat. No.: 216 008
Amount: 50 µg
Price: $420.00