Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Tyrosine hydroxylase antibody - 213 009

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis
Chicken monoclonal recombinant IgY
Cat. No.: 213 009
Amount: 50 µg
Price: $415.00
Cat. No. 213 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 10000 up to 1 : 20000 gallery  
IHC: 1 : 10000 up to 1 : 20000 (see remarks) gallery  
IHC-P: 1 : 5000 gallery  
Clone Ch103E1
Subtype IgY (λ light chain)
Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat TyrH (UniProt Id: P04177)
Epitop Epitop: AA 65 to 163 from rat TyrH (UniProt Id: P04177)
Reactivity Reacts with: mouse (P24529), rat (P04177).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 103E1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: This antibody is less sensitive than our polyclonal antibodies (cat. no. 213 102 and 213 104).
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining

Data sheet 213_009.pdf
Cat. No.: 213 009
Amount: 50 µg
Price: $415.00
Background
Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.