Tailor-made Antibodies
and Tools for Life Science

U1-70k antibody - 203 011

U1-70k is a member of the U1 snRNP
Mouse monoclonal purified IgG
Cat. No.: 203 011
Amount: 100 µg
Price: $420.00
Cat. No. 203 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: yes
IHC-P: 1 : 500 gallery  
FACS: yes
Clone H111
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 437 from human U1-70k (UniProt Id: P08621)
Epitop AA 1 to 275 from human U1-70k (UniProt Id: P08621)
Reactivity Reacts with: human (P08621), rat, mouse (Q62376), mammals.
Other species not tested yet.
Data sheet 203_011.pdf

References for U1-70k - 203 011

Autonomous transposons tune their sequences to ensure somatic suppression.
Ilık İA, Glažar P, Tse K, Brändl B, Meierhofer D, Müller FJ, Smith ZD, Aktaş T
Nature (2024) : . 203 011 WB; tested species: human
Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction.
Farshadyeganeh P, Nazim M, Zhang R, Ohkawara B, Nakajima K, Rahman MA, Nasrin F, Ito M, Takeda JI, Ohe K, Miyasaka Y, et al.
iScience (2023) 2610: 107746. 203 011 WB; tested species: mouse
Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcripts.
Mazille M, Buczak K, Scheiffele P, Mauger O
The EMBO journal (2022) : e110192. 203 011 WB; tested species: mouse
tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports (2020) 215: e49890. 203 011 WB, IP; tested species: mouse
Synergistic assembly of human pre-spliceosomes across introns and exons.
Braun JE, Friedman LJ, Gelles J, Moore MJ
eLife (2018) 7: . 203 011 WB; tested species: human
Post-transcriptional Regulation of De Novo Lipogenesis by mTORC1-S6K1-SRPK2 Signaling.
Lee G, Zheng Y, Cho S, Jang C, England C, Dempsey JM, Yu Y, Liu X, He L, Cavaliere PM, Chavez A, et al.
Cell (2017) 1717: 1545-1558.e18. 203 011 WB; tested species: human
Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Behm M, Wahlstedt H, Widmark A, Eriksson M, Öhman M
Journal of cell science (2017) 1304: 745-753. 203 011 WB; tested species: mouse
The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.
Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, Rajgor D, Kayikci M, Feltham J, Ule J, Eyras E, et al.
Nucleic acids research (2016) 4418: 8933-8950. 203 011 WB
A 10S galectin-3-U1 snRNP complex assembles into active spliceosomes.
Haudek KC, Voss PG, Wang JL, Patterson RJ
Nucleic acids research (2016) 4413: 6391-7. 203 011 WB
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
Rahman MA, Azuma Y, Nasrin F, Takeda J, Nazim M, Bin Ahsan K, Masuda A, Engel AG, Ohno K
Scientific reports (2015) 5: 13208. 203 011 WB
Detained introns are a novel, widespread class of post-transcriptionally spliced introns.
Boutz PL, Bhutkar A, Sharp PA
Genes & development (2015) 291: 63-80. 203 011 WB; tested species: mouse
A novel intra-U1 snRNP cross-regulation mechanism: alternative splicing switch links U1C and U1-70K expression.
Rösel-Hillgärtner TD, Hung LH, Khrameeva E, Le Querrec P, Gelfand MS, Bindereif A
PLoS genetics (2013) 910: e1003856. 203 011 WB; KD verified; tested species: human
Heterogeneous nuclear ribonucleoprotein A1 interferes with the binding of the human T cell leukemia virus type 1 rex regulatory protein to its response element.
Dodon MD, Hamaia S, Martin J, Gazzolo L
The Journal of biological chemistry (2002) 27721: 18744-52. 203 011 WB
Structure of the small nuclear RNP particle U1: identification of the two structural protuberances with RNP-antigens A and 70K.
Kastner B, Kornstädt U, Bach M, Lührmann R
The Journal of cell biology (1992) 1164: 839-49. 203 011 WB
Cat. No.: 203 011
Amount: 100 µg
Price: $420.00