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m6A - 202 111

Antibody against N6-methyladenosine modifications of RNA and DNA
Monoclonal mouse purified IgG
Cat. No.: 202 111
Amount: 100 µg
Price: $425.00
Cat. No. 202 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) suitable for Dot Blot gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes gallery  
Clone 212B11
Subtype IgG2b (κ light chain)
Immunogen N6-methyladenosine fused to BSA.
Reactivity Reacts with: human, rat, mouse, eukaryotes, prokaryotes.
Other species not tested yet.
Specificity Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am.
Data sheet 202_111.pdf

References for m6A - 202 111

Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.
Zong X, Wang H, Xiao X, Zhang Y, Hu Y, Wang F, Wang Y, Lu Z
RNA biology (2020) : 1-11. 202 111 DOTBLOT, IP; tested species: pig
Direct RNA sequencing enables m6A detection in endogenous transcript isoforms at base specific resolution.
Lorenz DA, Sathe S, Einstein JM, Yeo GW
RNA (New York, N.Y.) (2019) : . 202 111 DOTBLOT; tested species: human
Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System.
Weng YL, Wang X, An R, Cassin J, Vissers C, Liu Y, Liu Y, Xu T, Wang X, Wong SZH, Joseph J, et al.
Neuron (2018) 972: 313-325.e6. 202 111 DOTBLOT; tested species: mouse
Transcriptional Dysregulation in Postnatal Glutamatergic Progenitors Contributes to Closure of the Cortical Neurogenic Period.
Donega V, Marcy G, Lo Giudice Q, Zweifel S, Angonin D, Fiorelli R, Abrous DN, Rival-Gervier S, Koehl M, Jabaudon D, Raineteau O, et al.
Cell reports (2018) 2210: 2567-2574. 202 111 DOTBLOT
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.
Zong X, Wang H, Xiao X, Zhang Y, Hu Y, Wang F, Wang Y, Lu Z
RNA biology (2020) : 1-11. 202 111 DOTBLOT, IP; tested species: pig
YTHDF1-mediated translation amplifies Wnt-driven intestinal stemness.
Han B, Yan S, Wei S, Xiang J, Liu K, Chen Z, Bai R, Sheng J, Xu Z, Gao X
EMBO reports (2020) : e49229. 202 111 IP; tested species: mouse
NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination.
Werner S, Galliot A, Pichot F, Kemmer T, Marchand V, Sednev MV, Lence T, Roignant JY, König J, Höbartner C, Motorin Y, et al.
Nucleic acids research (2020) : . 202 111 IP; tested species: human,drosophila
Atlas of quantitative single-base-resolution N6-methyl-adenine methylomes.
Koh CWQ, Goh YT, Goh WSS
Nature communications (2019) 101: 5636. 202 111 IP; tested species: human
CCR7 Chemokine Receptor-Inducible lnc-Dpf3 Restrains Dendritic Cell Migration by Inhibiting HIF-1α-Mediated Glycolysis.
Liu J, Zhang X, Chen K, Cheng Y, Liu S, Xia M, Chen Y, Zhu H, Li Z, Cao X
Immunity (2019) 503: 600-615.e15. 202 111 IP; tested species: mouse
The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency.
Bertero A, Brown S, Madrigal P, Osnato A, Ortmann D, Yiangou L, Kadiwala J, Hubner NC, de Los Mozos IR, Sadée C, Lenaerts AS, et al.
Nature (2018) 5557695: 256-259. 202 111 IP; tested species: human
Human ALKBH3-induced m1A demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells.
Woo HH, Chambers SK
Biochimica et biophysica acta. Gene regulatory mechanisms (2018) : . 202 111 IP; tested species: human
Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones.
Vysokov NV, Silva JP, Lelianova VG, Suckling J, Cassidy J, Blackburn JK, Yankova N, Djamgoz MB, Kozlov SV, Tonevitsky AG, Ushkaryov YA, et al.
eLife (2018) 7: . 202 111 IP
Determination of enrichment factors for modified RNA in MeRIP experiments.
Slama K, Galliot A, Weichmann F, Hertler J, Feederle R, Meister G, Helm M
Methods (San Diego, Calif.) (2018) : . 202 111 IP
Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.
Warda AS, Kretschmer J, Hackert P, Lenz C, Urlaub H, Höbartner C, Sloan KE, Bohnsack MT
EMBO reports (2017) 1811: 2004-2014. 202 111 IP; tested species: human
Epitranscriptomic Enhancement of Influenza A Virus Gene Expression and Replication.
Courtney DG, Kennedy EM, Dumm RE, Bogerd HP, Tsai K, Heaton NS, Cullen BR
Cell host & microbe (2017) 223: 377-386.e5. 202 111 IP; tested species: human
N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection.
Gokhale NS, McIntyre ABR, McFadden MJ, Roder AE, Kennedy EM, Gandara JA, Hopcraft SE, Quicke KM, Vazquez C, Willer J, Ilkayeva OR, et al.
Cell host & microbe (2016) 205: 654-665. 202 111 IP
Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB
Nature structural & molecular biology (2016) 231: 24-30. 202 111 IP
Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 111 IP
N6-Adenosine Methylation in RNA and a Reduced m3G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions.
Svobodová Kovaříková A, Stixová L, Kovařík A, Komůrková D, Legartová S, Fagherazzi P, Bártová E
Cells (2020) 92: . 202 111 ICC; tested species: mouse
Cat. No.: 202 111
Quantity: 100 µg
Price: $425.00
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.
Zong X, Wang H, Xiao X, Zhang Y, Hu Y, Wang F, Wang Y, Lu Z
RNA biology (2020) : 1-11. 202 111 DOTBLOT, IP; tested species: pig
Direct RNA sequencing enables m6A detection in endogenous transcript isoforms at base specific resolution.
Lorenz DA, Sathe S, Einstein JM, Yeo GW
RNA (New York, N.Y.) (2019) : . 202 111 DOTBLOT; tested species: human
Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System.
Weng YL, Wang X, An R, Cassin J, Vissers C, Liu Y, Liu Y, Xu T, Wang X, Wong SZH, Joseph J, et al.
Neuron (2018) 972: 313-325.e6. 202 111 DOTBLOT; tested species: mouse
Transcriptional Dysregulation in Postnatal Glutamatergic Progenitors Contributes to Closure of the Cortical Neurogenic Period.
Donega V, Marcy G, Lo Giudice Q, Zweifel S, Angonin D, Fiorelli R, Abrous DN, Rival-Gervier S, Koehl M, Jabaudon D, Raineteau O, et al.
Cell reports (2018) 2210: 2567-2574. 202 111 DOTBLOT
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.
Zong X, Wang H, Xiao X, Zhang Y, Hu Y, Wang F, Wang Y, Lu Z
RNA biology (2020) : 1-11. 202 111 DOTBLOT, IP; tested species: pig
YTHDF1-mediated translation amplifies Wnt-driven intestinal stemness.
Han B, Yan S, Wei S, Xiang J, Liu K, Chen Z, Bai R, Sheng J, Xu Z, Gao X
EMBO reports (2020) : e49229. 202 111 IP; tested species: mouse
NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination.
Werner S, Galliot A, Pichot F, Kemmer T, Marchand V, Sednev MV, Lence T, Roignant JY, König J, Höbartner C, Motorin Y, et al.
Nucleic acids research (2020) : . 202 111 IP; tested species: human,drosophila
Atlas of quantitative single-base-resolution N6-methyl-adenine methylomes.
Koh CWQ, Goh YT, Goh WSS
Nature communications (2019) 101: 5636. 202 111 IP; tested species: human
CCR7 Chemokine Receptor-Inducible lnc-Dpf3 Restrains Dendritic Cell Migration by Inhibiting HIF-1α-Mediated Glycolysis.
Liu J, Zhang X, Chen K, Cheng Y, Liu S, Xia M, Chen Y, Zhu H, Li Z, Cao X
Immunity (2019) 503: 600-615.e15. 202 111 IP; tested species: mouse
The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency.
Bertero A, Brown S, Madrigal P, Osnato A, Ortmann D, Yiangou L, Kadiwala J, Hubner NC, de Los Mozos IR, Sadée C, Lenaerts AS, et al.
Nature (2018) 5557695: 256-259. 202 111 IP; tested species: human
Human ALKBH3-induced m1A demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells.
Woo HH, Chambers SK
Biochimica et biophysica acta. Gene regulatory mechanisms (2018) : . 202 111 IP; tested species: human
Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones.
Vysokov NV, Silva JP, Lelianova VG, Suckling J, Cassidy J, Blackburn JK, Yankova N, Djamgoz MB, Kozlov SV, Tonevitsky AG, Ushkaryov YA, et al.
eLife (2018) 7: . 202 111 IP
Determination of enrichment factors for modified RNA in MeRIP experiments.
Slama K, Galliot A, Weichmann F, Hertler J, Feederle R, Meister G, Helm M
Methods (San Diego, Calif.) (2018) : . 202 111 IP
Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.
Warda AS, Kretschmer J, Hackert P, Lenz C, Urlaub H, Höbartner C, Sloan KE, Bohnsack MT
EMBO reports (2017) 1811: 2004-2014. 202 111 IP; tested species: human
Epitranscriptomic Enhancement of Influenza A Virus Gene Expression and Replication.
Courtney DG, Kennedy EM, Dumm RE, Bogerd HP, Tsai K, Heaton NS, Cullen BR
Cell host & microbe (2017) 223: 377-386.e5. 202 111 IP; tested species: human
N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection.
Gokhale NS, McIntyre ABR, McFadden MJ, Roder AE, Kennedy EM, Gandara JA, Hopcraft SE, Quicke KM, Vazquez C, Willer J, Ilkayeva OR, et al.
Cell host & microbe (2016) 205: 654-665. 202 111 IP
Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB
Nature structural & molecular biology (2016) 231: 24-30. 202 111 IP
Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 111 IP
N6-Adenosine Methylation in RNA and a Reduced m3G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions.
Svobodová Kovaříková A, Stixová L, Kovařík A, Komůrková D, Legartová S, Fagherazzi P, Bártová E
Cells (2020) 92: . 202 111 ICC; tested species: mouse
Background
m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.