|Cat. No. 202 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 , suitable for Dot-Blot
IP: yes (see remarks)
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
Reacts with: mouse, rat, human, mammals, eukaryotes, prokaryotes.
Other species not tested yet.
|Specificity||Does not discriminate between Adenosin and N6-methyladenosine|
IP: This antibody is a suitable control for m6A sequencing.
m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.
This antibody does not discriminate between modified and unmodified Adenosine and can be used as an internal control.