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Adenosine - 202 103

Antibody against adenosine of RNA and DNA
Polyclonal rabbit purified antibody
Cat. No.: 202 103
Amount: 50 µg
Price: $370.00
Cat. No. 202 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 , suitable for Dot-Blot
IP: yes (see remarks)
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes
Reactivity Reacts with: mouse, rat, human, mammals, eukaryotes, prokaryotes.
Other species not tested yet.
Specificity Does not discriminate between Adenosin and N6-methyladenosine
Remarks

IP: This antibody is a suitable control for m6A sequencing.

Data sheet 202_103.pdf
Cat. No.: 202 103
Quantity: 50 µg
Price: $370.00
Background

m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.
This antibody does not discriminate between modified and unmodified Adenosine and can be used as an internal control.