Synaptic Systems - Adenosine

Background

m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.
This antibody does not discriminate between modified and unmodified Adenosine and can be used as an internal control.

1 ) Bringmann P et al., FEBS Lett. (1987)
2 ) Warda AS et al., EMBO Rep. (2017)
3 ) Xiang Y et al., Nature (2017)
4 ) Koziol MJ et al., Bio Protoc (2016)
5 ) Koziol MJ et al., Nat. Struct. Mol. Biol. (2016)
6 ) Gokhale NS et al., Cell Host Microbe (2016)
7 ) Li X et al., Int J Mol Sci (2016)
8 ) Molinie B et al., Nat. Methods (2016)
9 ) Mishima E et al., PLoS ONE (2015)
10 ) Li Y et al., RNA (2015)

Cat. No. 202 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 , suitable for Dot-Blot IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P: not tested yet ELISA: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Reactivity	Reacts with: mouse, rat, human, mammals, eukaryotes, prokaryotes. Other species not tested yet.
Specificity	Does not discriminate between Adenosin and N6-methyladenosine
Remarks	IP: This antibody is a suitable control for m6A sequencing.
Data sheet	202_103.pdf

Adenosine antibody - 202 103