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GAD 1 / GAD 67 antibody - 198 211

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Monoclonal mouse purified IgG
Cat. No.: 198 211
Amount: 100 µg
Price: $415.00
Cat. No. 198 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500      gallery  
IHC-P/FFPE: 1 : 1000      gallery  
Clone 126G12
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 101 from mouse GAD1 (UniProt Id: P48318)
Epitop Epitop: AA 94 to 101 from mouse GAD1 (UniProt Id: P48318)
Reactivity Reacts with: mouse (P48318), rat (P18088).
Other species not tested yet.
Specificity Specific for GAD 1 / GAD67.
Remarks

This antibody is highly recommened for Western blot and IF.

Data sheet 198_211.pdf

References for GAD 1 / GAD 67 - 198 211

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 211 ICC; tested species: human
Atlastin-1 modulates seizure activity and neuronal excitability.
Lu X, Yang M, Yang Y, Wang XF
CNS neuroscience & therapeutics (2019) : . 198 211 IHC; tested species: human,mouse
Cat. No.: 198 211
Quantity: 100 µg
Price: $415.00
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 211 ICC; tested species: human
Atlastin-1 modulates seizure activity and neuronal excitability.
Lu X, Yang M, Yang Y, Wang XF
CNS neuroscience & therapeutics (2019) : . 198 211 IHC; tested species: human,mouse
Background

The glutamic acid decarboxylases GAD 1, also referred to as GAD 67, and GAD 2 / GAD 65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.