Parvalbumin antibody - 195 011C3

Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones

Mouse monoclonal purified IgG

Cat. No.: 195 011C3

Amount: 100 µg

Price: $465.00

Cat. No. 195 011C3	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Sulfo-Cyanine 3
Clone	58E1
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity	Reacts with: rat (P02625), mouse (P32848). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	195-0P
Data sheet	195_011c3.pdf

References for Parvalbumin - 195 011C3

Brain volumetric alterations accompanied with loss of striatal medium-sized spiny neurons and cortical parvalbumin expressing interneurons in Brd1+/- mice.
Qvist P, Eskildsen SF, Hansen B, Baragji M, Ringgaard S, Roovers J, Paternoster V, Molgaard S, Corydon TJ, Stødkilde-Jørgensen H, Glerup S, et al.
Scientific reports (2018) 81: 16486. 195 011C3 IHC; tested species: mouse

Cat. No.: 195 011C3

Amount: 100 µg

Price: $465.00

IHC

Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.

1 ) Andrioli A et al., Neuroscience (2007)
2 ) Hendrickson A et al., Exp. Eye Res. (2007)
3 ) Fukuda T et al., Neuroscience (2003)
4 ) Kosaka K et al., Exp Brain Res (1994)
5 ) Tolosa de Talamoni N et al., Proc. Natl. Acad. Sci. U.S.A. (1993)
6 ) Kubota Y et al., Neuroreport (1993)

Protocols

IHC

OTHER

Preabsorbtion

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