Parvalbumin antibody - 195 011C2

Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones

Mouse monoclonal purified IgG

Cat. No.: 195 011C2

Amount: 100 µg

Price: $465.00

Cat. No. 195 011C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: not tested yet IHC: 1 : 200 up to 1 : 50 gallery IHC-P: not tested yet
Label	Cyanine 2
Clone	58E1
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity	Reacts with: rat (P02625), mouse (P32848). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	195-0P
Data sheet	195_011c2.pdf

References for Parvalbumin - 195 011C2

Loss of Hippocampal Calretinin and Parvalbumin Interneurons in the 5XFAD Mouse Model of Alzheimer's Disease.
Giesers NK, Wirths O
ASN neuro () 12: 1759091420925356. 195 011C2 IHC; tested species: mouse

Cat. No.: 195 011C2

Amount: 100 µg

Price: $465.00

IHC

Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.

1 ) Andrioli A, Neuroscience (2007) 1491: 131-43.
2 ) Hendrickson A, Experimental eye research (2007) 855: 587-601.
3 ) Fukuda T, Neuroscience (2003) 1201: 5-20.
4 ) Kosaka K, Experimental brain research (1994) 992: 205-13.
5 ) Tolosa de Talamoni N, Proceedings of the National Academy of Sciences of the United States of America (1993) 9024: 11949-53.

Protocols

IHC

OTHER

Preabsorbtion

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