Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Ribeye antibody A-domain - 192 104

Ribeye is a scaffolding protein of the ribbon synapse
Guinea pig polyclonal antiserum
Cat. No.: 192 104
Amount: 100 µl
Price: $365.00
Cat. No. 192 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 10000 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Immunogen Recombinant protein corresponding to AA 95 to 207 from rat Ribeye (UniProt Id: Q9EQH5-2)
Reactivity Reacts with: rat (Q9EQH5-2), mouse (P56546-2).
Other species not tested yet.
Specificity This antibody recognizes only ribeye and not CtBP 2.
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 or Klotz et al. 2019 is recommended.

Data sheet 192_104.pdf

References for Ribeye - 192 104

Hyperacusis in the Adult Fmr1-KO Mouse Model of Fragile X Syndrome: The Therapeutic Relevance of Cochlear Alterations and BKCa Channels.
Ferraguto C, Bouleau Y, Peineau T, Dulon D, Pietropaolo S
International journal of molecular sciences (2023) 2414: . 192 104 IHC; tested species: mouse
Glutamate transporters EAAT2 and EAAT5 differentially shape synaptic transmission from rod bipolar cell terminals.
Tang FS, Yuan HL, Liu JB, Zhang G, Chen SY, Ke JB
eNeuro (2022) : . 192 104 IHC; tested species: mouse
The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.
Calvet C, Peineau T, Benamer N, Cornille M, Lelli A, Plion B, Lahlou G, Fanchette J, Nouaille S, Boutet de Monvel J, Estivalet A, et al.
iScience (2022) 2512: 105628. 192 104 IHC; tested species: mouse
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 192 104 IHC; tested species: mouse
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 192 104 IHC; tested species: mouse
Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses.
Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201901543R. 192 104 IHC; tested species: mouse
Cat. No.: 192 104
Amount: 100 µl
Price: $365.00
Hyperacusis in the Adult Fmr1-KO Mouse Model of Fragile X Syndrome: The Therapeutic Relevance of Cochlear Alterations and BKCa Channels.
Ferraguto C, Bouleau Y, Peineau T, Dulon D, Pietropaolo S
International journal of molecular sciences (2023) 2414: . 192 104 IHC; tested species: mouse
Glutamate transporters EAAT2 and EAAT5 differentially shape synaptic transmission from rod bipolar cell terminals.
Tang FS, Yuan HL, Liu JB, Zhang G, Chen SY, Ke JB
eNeuro (2022) : . 192 104 IHC; tested species: mouse
The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.
Calvet C, Peineau T, Benamer N, Cornille M, Lelli A, Plion B, Lahlou G, Fanchette J, Nouaille S, Boutet de Monvel J, Estivalet A, et al.
iScience (2022) 2512: 105628. 192 104 IHC; tested species: mouse
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 192 104 IHC; tested species: mouse
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 192 104 IHC; tested species: mouse
Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses.
Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201901543R. 192 104 IHC; tested species: mouse
Background

The photoreceptor ribbon synapse is a unique type of synapse specialized for the tonic release of neurotransmitter in the dark. Ribeye is a self-aggregating protein and is one of the major scaffolding components of the ribbon on which the neurotransmitter containing vesicles are tethered. The protein consists of a unique A-domain and a B-domain. With the exception of the first 20 amino acids the B-domain is identical to the transcriptional corepressor CtBP 2. Both proteins originate from the same gene.