mGluR6 antibody - 191 603

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission

Rabbit polyclonal purified antibody

Cat. No.: 191 603

Amount: 50 µg

Price: $380.00

Cat. No. 191 603	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 5000 (see remarks) gallery IHC-P: not recommended IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse mGluR6 (UniProt Id: Q5NCH9)
Reactivity	Reacts with: mouse (Q5NCH9), rat (P35349). Other species not tested yet.
Remarks	IHC: For optimal results in retina tissue, follow the retina protocol. IHC-Fr: Acetone fixation is recommended. The following fixatives are not advised: methanol-acetone, 4% formaldehyde/PFA, methanol.
Data sheet	191_603.pdf

Cat. No.: 191 603

Amount: 50 µg

Price: $380.00

Immunofluorescence image of a mouse retina stained for mGluR6

IHC

IHC-Fr

Background

Glutamate is the main excitatory neurotransmitter in the vertebrate nervous system and regulates a number of cellular signaling pathways. In addition to the ionotropic glutamate receptors of the NMDA, AMPA and kainate type, eight metabotropic receptors (mGluR1-8) have been described so far (1). This receptor family can be subdivided into three groups (2): Group I receptors (mGluR1, mGluR5) are activated by dihydroxyphenylglycine (DHPG) (3), group II receptors (mGluR2, mGluR3) by dicarboxycyclopropylglycine (DCG-IV), and group III receptors (mGluR4, mGluR6, mGluR7, mGluR8) by aminophosphonobutyrate (L-AP4) (4).
mGluR6 is involved in glutamate transmission from photoreceptors and is concentrated at postsynaptic sites of rod bipolar cells in the retina (5).

1 ) Shigemoto R et al., J Neurosci (1997)
2 ) Nakanishi S et al., Annu Rev Biophys Biomol Struct (1994)
3 ) Schoepp DD et al., J Neurochem (1994)
4 ) Hayashi Y et al., Nature (1993)
5 ) Nomura A et al., Cell (1994)

Protocols

IHC

IHC-Fr