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mGluR1-alpha antibody - 191 003

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 191 003
Amount: 50 µg
Price: $455.00
Cat. No. 191 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (AP-staining) gallery  
ICC: yes gallery  
IHC: (see remarks) gallery  
IHC-P: yes gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse mGluR1-α (UniProt Id: P97772)
Reactivity Reacts with: mouse (P97772), rat (P23385).
Other species not tested yet.
Specificity This antibody is specific for mGluR1-α and shows no cross-reactivity to mGluR1-β
Remarks

IHC: For best results, heat-mediated antigen retrieval (citrate buffer pH 6) is recommended.

Data sheet 191_003.pdf
Cat. No.: 191 003
Amount: 50 µg
Price: $455.00
Background

Glutamate is the main excitatory neurotransmitter in the vertebrate nervous system and regulates a number of cellular signaling pathways. In addition to the ionotropic glutamate receptors of the NMDA, AMPA and kainate type, eight metabotropic receptors (mGluR1-8) have been described so far (1). This receptor family can be subdivided into three groups (2): Group I receptors (mGluR1, mGluR5) are activated by dihydroxyphenylglycine (DHPG) (3), group II receptors (mGluR2, mGluR3) by dicarboxycyclopropylglycine (DCG-IV), and group III receptors (mGluR4, mGluR6, mGluR7, mGluR8) by aminophosphonobutyrate (L-AP4) (4).