Densin180 antibody - 189 002

Densin180 is a PSD protein

Rabbit polyclonal antiserum

Cat. No.: 189 002

Amount: 200 µl

Price: $355.00

Cat. No. 189 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 877 to 891 and 1341 to 1357 from rat Densin 180 (UniProt Id: P70587)
Reactivity	Reacts with: rat (P70587), mouse (Q80TE7). Other species not tested yet.
Remarks	The crude antiserum is recommended for WB (stronger signals). It is not recommended for ICC.
Data sheet	189_002.pdf

Cat. No.: 189 002

Amount: 200 µl

Price: $355.00

Background

Densin is a 180 kDa transmembrane glycoprotein which belongs to the LAP family [leucine-rich repeat (LRR) and PDZ] family, characterized by an LRR near the N-terminus and one or more PDZ domains at the C-terminus. Several developmentally regulated splice variants and protein-protein interactions with CamKII and nephrin have been described. The protein locates to the post-synaptic density (PSD) in nerve cells but has also been shown to be expressed in glomerular podocytes in kidney.

1 ) Ahola H et al., J. Am. Soc. Nephrol. (2003)
2 ) Walikonis RS et al., J. Neurosci. (2001)
3 ) Strack S et al., J. Biol. Chem. (2000)
4 ) Apperson ML et al., J. Neurosci. (1996)

Protocols

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