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Home > Products > 185 002

CASKIN1 antibody - 185 002

CASKIN is a CASK interaction partner
Rabbit polyclonal antiserum
Cat. No.: 185 002
Amount: 200 µl
Price: $355.00
Cat. No. 185 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: yes
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 1416 to 1430 from rat CASKIN1 (UniProt Id: Q8VHK2)
Reactivity Reacts with: human (Q8WXD9), rat (Q8VHK2), mouse (Q6P9K8), cow, dog, monkey.
Other species not tested yet.
Specificity Specific for CASKIN 1.The epiotope is present in splice variants 1, 2, 3 but missing in variant 4.
Matching control protein/peptide 185-0P
Data sheet 185_002.pdf

References for CASKIN1 - 185 002

miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1In vivoandvitro.
Lv X, Zhao K, Lan Y, Li Z, Ding N, Su J, Lu H, Song D, Gao F, He W
Frontiers in microbiology (2017) 8: 304. 185 002 WB; tested species: mouse
Differential synaptic distribution of the scaffold proteins Cask and Caskin1 in the bovine retina.
Anjum R, Ayoubian H, Schmitz F
Molecular and cellular neurosciences (2014) 62: 19-29. 185 002 WB, IHC; tested species: cow
Differential synaptic distribution of the scaffold proteins Cask and Caskin1 in the bovine retina.
Anjum R, Ayoubian H, Schmitz F
Molecular and cellular neurosciences (2014) 62: 19-29. 185 002 WB, IHC; tested species: cow
Cat. No.: 185 002
Amount: 200 µl
Price: $355.00
miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1In vivoandvitro.
Lv X, Zhao K, Lan Y, Li Z, Ding N, Su J, Lu H, Song D, Gao F, He W
Frontiers in microbiology (2017) 8: 304. 185 002 WB; tested species: mouse
Differential synaptic distribution of the scaffold proteins Cask and Caskin1 in the bovine retina.
Anjum R, Ayoubian H, Schmitz F
Molecular and cellular neurosciences (2014) 62: 19-29. 185 002 WB, IHC; tested species: cow
Differential synaptic distribution of the scaffold proteins Cask and Caskin1 in the bovine retina.
Anjum R, Ayoubian H, Schmitz F
Molecular and cellular neurosciences (2014) 62: 19-29. 185 002 WB, IHC; tested species: cow
WB
ICC
Background

The complex of the multi-adaptor-proteins CASK, Veli and Mint is conserved across kingdoms. CASKIN 1 is a novel CASK interaction partner that binds to its Cam Kinase domain and competes with Mint so that alternative complexes can be formed. Four different splice variants have been identified so far.
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