Tailor-made Antibodies
and Tools for Life Science

GluA antibody extracellular - 182 411C3

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission
Mouse monoclonal purified IgG
Cat. No.: 182 411C3
Amount: 100 µg
Price: $470.00
Cat. No. 182 411C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.
Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 248B7
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to the extracellular amino-terminus of rat GluA2. (UniProt Id: P19491)
Reactivity Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8).
Other species not tested yet.
Specificity Raised against GluA2 but detects GluA1, 2, and 3 transfected cells with a strong preference for GluA1. Due to sequence homology, it likely crossreacts also to GluA4.
Remarks

ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.

Data sheet 182_411c3.pdf

References for GluA - 182 411C3

Rasopathy-Associated Mutation Ptpn11D61Y has Age-Dependent Effect on Synaptic Vesicle Recycling.
Guhathakurta D, Selzam F, Petrušková A, Weiss EM, Akdaş EY, Montenegro-Venegas C, Zenker M, Fejtová A
Cellular and molecular neurobiology (2024) 441: 77. 182 411C3 ICC; tested species: mouse
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 182 411C3 ICC; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 182 411C3 ICC; tested species: rat
Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 182 411C3 ICC; tested species: mouse
Cat. No.: 182 411C3
Amount: 100 µg
Price: $470.00