Synaptic Systems

Cat. No.: 182 411C3

Amount: 100 µg

Price: $465.00

Cat. No. 182 411C3	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: not recommended IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Sulfo-Cyanine 3
Clone	248B7
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to the extracellular amino-terminus of rat GluA2. (UniProt Id: P19491)
Reactivity	Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8). Other species not tested yet.
Specificity	Raised against GluA2 but detects GluA1, 2, and 3 transfected cells with a strong preference for GluA1. Due to sequence homology, it likely crossreacts also to GluA4.
Remarks	ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.
Data sheet	182_411c3.pdf

References for GluA - 182 411C3

Rasopathy-Associated Mutation Ptpn11D61Y has Age-Dependent Effect on Synaptic Vesicle Recycling.
Guhathakurta D, Selzam F, Petrušková A, Weiss EM, Akdaş EY, Montenegro-Venegas C, Zenker M, Fejtová A
Cellular and molecular neurobiology (2024) 441: 77. 182 411C3 ICC; tested species: mouse

CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 182 411C3 ICC; tested species: mouse

Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 182 411C3 ICC; tested species: rat

Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 182 411C3 ICC; tested species: mouse

Cat. No.: 182 411C3

Amount: 100 µg

Price: $465.00

ICC

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Deng YP et al., J. Chem. Neuroanat. (2007)
4 ) Steiner P et al., EMBO J. (2005)
5 ) Nagy GG et al., J. Neurosci. (2004)
6 ) Passafaro M et al., Nature (2003)
7 ) Iihara K et al., J. Neurosci. (2001)
8 ) Daw MI et al., Neuron (2000)
9 ) Osten P et al., Neuron (1998)
10 ) He Y et al., Exp. Neurol. (1998)

Protocols

ICC

Other products for this target

GluA antibody extracellular - 182 411C3

References for GluA - 182 411C3