Tailor-made Antibodies
and Tools for Life Science

GluA antibody extracellular - 182 411

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission
Mouse monoclonal purified IgG
Cat. No.: 182 411
Amount: 100 µg
Price: $425.00
Cat. No. 182 411 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: yes
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Clone 248B7
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to the extracellular amino-terminus of rat GluA2. (UniProt Id: P19491)
Reactivity Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8), human (P42262).
Other species not tested yet.
Specificity Raised against GluA2 but detects GluA1, 2, and 3 transfected cells with a strong preference for GluA1. Due to sequence homology, it likely crossreacts also to GluA4.
Remarks

ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.

Data sheet 182_411.pdf

References for GluA - 182 411

A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors.
Boudkkazi S, Schwenk J, Nakaya N, Brechet A, Kollewe A, Harada H, Bildl W, Kulik A, Dong L, Sultana A, Zolles G, et al.
Neuron (2023) 11116: 2544-2556.e9. 182 411 IP; tested species: mouse
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 411 IP; tested species: mouse
Cat. No.: 182 411
Amount: 100 µg
Price: $425.00