Synaptic Systems

Cat. No.: 182 411

Amount: 100 µg

Price: $420.00

Cat. No. 182 411	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	248B7
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to the extracellular amino-terminus of rat GluA2. (UniProt Id: P19491)
Reactivity	Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8), human (P42262). Other species not tested yet.
Specificity	Raised against GluA2 but detects GluA1, 2, and 3 transfected cells with a strong preference for GluA1. Due to sequence homology, it likely crossreacts also to GluA4.
Remarks	ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.
Data sheet	182_411.pdf

References for GluA - 182 411

A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors.
Boudkkazi S, Schwenk J, Nakaya N, Brechet A, Kollewe A, Harada H, Bildl W, Kulik A, Dong L, Sultana A, Zolles G, et al.
Neuron (2023) 11116: 2544-2556.e9. 182 411 IP; tested species: mouse

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 411 IP; tested species: mouse

LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface.
Liouta K, Lubas M, Venugopal V, Chabbert J, Jeannière C, Diaz C, Munier M, Tessier B, Claverol S, Favereaux A, Sainlos M, et al.
Nature communications (2024) 151: 8807. 182 411 ICC; tested species: mouse

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity.
van Oostrum M, Campbell B, Seng C, Müller M, Tom Dieck S, Hammer J, Pedrioli PGA, Földy C, Tyagarajan SK, Wollscheid B
Nature communications (2020) 111: 4990. 182 411 ICC; tested species: rat

Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 182 411 ICC; tested species: mouse

The Role of Agrin, Lrp4 and MuSK during Dendritic Arborization and Synaptogenesis in Cultured Embryonic CNS Neurons.
Handara G, Hetsch FJA, Jüttner R, Schick A, Haupt C, Rathjen FG, Kröger S
Developmental biology (2018) : . 182 411 ICC; tested species: mouse

Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 182 411 ICC

miR-124-dependent tagging of synapses by synaptopodin enables input-specific homeostatic plasticity.
Dubes S, Soula A, Benquet S, Tessier B, Poujol C, Favereaux A, Thoumine O, Letellier M
The EMBO journal (2022) : e109012. 182 411 UPTAKE; tested species: rat

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons.
Bessa-Neto D, Beliu G, Kuhlemann A, Pecoraro V, Doose S, Retailleau N, Chevrier N, Perrais D, Sauer M, Choquet D
Nature communications (2021) 121: 6715. 182 411 UPTAKE; tested species: rat

Cat. No.: 182 411

Amount: 100 µg

Price: $420.00

ICC

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Deng YP et al., J. Chem. Neuroanat. (2007)
4 ) Steiner P et al., EMBO J. (2005)
5 ) Nagy GG et al., J. Neurosci. (2004)
6 ) Passafaro M et al., Nature (2003)
7 ) Iihara K et al., J. Neurosci. (2001)
8 ) Daw MI et al., Neuron (2000)
9 ) Osten P et al., Neuron (1998)
10 ) He Y et al., Exp. Neurol. (1998)

Protocols

Standard protocol

ICC

Other products for this target

GluA antibody extracellular - 182 411

References for GluA - 182 411