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GluA - 182 411

A member of a receptor group involved in rapid excitatory neurotransmission in the mammalian CNS
Monoclonal mouse purified IgG
Cat. No.: 182 411
Amount: 100 µg
Price: 415.00 €
Cat. No. 182 411 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone 248B7
Subtype IgG2a (κ light chain)
Immunogen Nativ Protein corresponding to AA 22 to 545 from rat GluA2 (UniProt Id: P19491)
Epitop Epitop: AA 22 to 545 from rat GluA2 (UniProt Id: P19491)
Reactivity Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8).
Other species not tested yet.
Specificity Raised against GluA 2 but detects GluA 1, 2, and 3 transfected cells. Due to sequence homology, it likely crossreacts also to GluA 4.
Remarks

ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.

Data sheet 182_411.pdf

References for GluA - 182 411

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 411 IP; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 182 411 ICC; tested species: mouse
The Role of Agrin, Lrp4 and MuSK during Dendritic Arborization and Synaptogenesis in Cultured Embryonic CNS Neurons.
Handara G, Hetsch FJA, Jüttner R, Schick A, Haupt C, Rathjen FG, Kröger S
Developmental biology (2018) : . 182 411 ICC; tested species: mouse
Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 182 411 ICC
Cat. No.: 182 411
Quantity: 100 µg
Price: 415.00 €
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 411 IP; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 182 411 ICC; tested species: mouse
The Role of Agrin, Lrp4 and MuSK during Dendritic Arborization and Synaptogenesis in Cultured Embryonic CNS Neurons.
Handara G, Hetsch FJA, Jüttner R, Schick A, Haupt C, Rathjen FG, Kröger S
Developmental biology (2018) : . 182 411 ICC; tested species: mouse
Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 182 411 ICC
Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

Protocols