Synaptic Systems

Cat. No.: 182 203

Amount: 50 µg

Price: $375.00

Cat. No. 182 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 860 to 871 from mouse GluA3 (UniProt Id: Q9Z2W9)
Reactivity	Reacts with: human (P42263), rat (P19492), mouse (Q9Z2W9), pig, cow. No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	182-2P
Data sheet	182_203.pdf

References for GluA3 - 182 203

Chronic mild corticosterone exposure during adolescence enhances behaviors and upregulates neuroplasticity-related proteins in rat hippocampus.
Li J, Li Y, Sun Y, Wang H, Liu X, Zhao Y, Wang H, Su Y, Si T
Progress in neuro-psychopharmacology & biological psychiatry (2019) 89: 400-411. 182 203 WB; tested species: rat

Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice.
Sultana A, Nakaya N, Dong L, Abu-Asab M, Qian H, Tomarev SI
Experimental neurology (2014) 261: 802-11. 182 203 WB

Deletion in the N-terminal half of olfactomedin 1 modifies its interaction with synaptic proteins and causes brain dystrophy and abnormal behavior in mice.
Nakaya N, Sultana A, Munasinghe J, Cheng A, Mattson MP, Tomarev SI
Experimental neurology (2013) 250: 205-18. 182 203 WB; tested species: mouse

Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 203 WB

GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites.
Perozzo AM, Schwenk J, Kamalova A, Nakagawa T, Fakler B, Bowie D
Nature communications (2023) 141: 6799. 182 203 IP; tested species: rat

AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability.
Brechet A, Buchert R, Schwenk J, Boudkkazi S, Zolles G, Siquier-Pernet K, Schaber I, Bildl W, Saadi A, Bole-Feysot C, Nitschke P, et al.
Nature communications (2017) 8: 15910. 182 203 IP; tested species: mouse

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Schwenk J, Harmel N, Brechet A, Zolles G, Berkefeld H, Müller CS, Bildl W, Baehrens D, Hüber B, Kulik A, Klöcker N, et al.
Neuron (2012) 744: 621-33. 182 203 IP; tested species: rat

Molecular Characterization of AMPA-Receptor-Containing Vesicles.
Peters JJ, Leitz J, Oses-Prieto JA, Burlingame AL, Brunger AT
Frontiers in molecular neuroscience (2021) 14: 754631. 182 203 EM; tested species: mouse

Cat. No.: 182 203

Amount: 50 µg

Price: $375.00

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Meng Y et al., Neuron (2003)
3 ) Moga DE et al., J. Comp. Neurol. (2003)
4 ) Frassoni C et al., Neurology (2001)
5 ) Breese CR et al., J. Mol. Neurosci. (1996)
6 ) Ginsberg SD et al., Neuroscience (1995)
7 ) Keller BU et al., EMBO J. (1992)
8 ) Hughes TE et al., Vis. Neurosci. (1992)

Protocols

Standard protocol

OTHER

Preabsorbtion

Other products for this target

GluA3 antibody - 182 203

References for GluA3 - 182 203