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Home > Products > 182 111

GluA2 antibody - 182 111

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission
Mouse monoclonal purified IgG
Cat. No.: 182 111
Amount: 100 µg
Price: $420.00
Cat. No. 182 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
ExM: yes (see remarks) gallery  
Clarity: 1 : 200 (see remarks)
Clone 1404A10
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 278 to 292 from rat GluA2 (UniProt Id: P19491)
Reactivity Reacts with: rat (P19491), mouse (P23819), human (P42262).
No signal: zebrafish.
Other species not tested yet.
Remarks

IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet 182_111.pdf

References for GluA2 - 182 111

SHANK2 Mutations Result in Dysregulation of the ERK1/2 Pathway in Human Induced Pluripotent Stem Cells-Derived Neurons and Shank2(-/-) Mice.
Lutz AK, Pérez Arévalo A, Ioannidis V, Stirmlinger N, Demestre M, Delorme R, Bourgeron T, Boeckers TM
Frontiers in molecular neuroscience (2021) 14: 773571. 182 111 WB, ICC; tested species: human
Heterogeneity of Cell Surface Glutamate and GABA Receptor Expression in Shank and CNTN4 Autism Mouse Models.
Heise C, Preuss JM, Schroeder JC, Battaglia CR, Kolibius J, Schmid R, Kreutz MR, Kas MJH, Burbach JPH, Boeckers TM
Frontiers in molecular neuroscience (2018) 11: 212. 182 111 WB; tested species: mouse
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 182 111 WB, ICC; tested species: rat
Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo.
Jedlicka P, Vnencak M, Krueger DD, Jungenitz T, Brose N, Schwarzacher SW
Brain structure & function (2015) 2201: 47-58. 182 111 WB
Cat. No.: 182 111
Amount: 100 µg
Price: $420.00
WB
ExM
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