Synaptic Systems

Cat. No.: 182 105

Amount: 50 µg

Price: $465.00

Cat. No. 182 105	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 836 to 883 from rat GluA2 (UniProt Id: P19491)
Reactivity	Reacts with: rat (P19491), mouse. Other species not tested yet.
Specificity	Some cross reactivity to GluA 3.
Matching control protein/peptide	182-1P
Data sheet	182_105.pdf

References for GluA2 - 182 105

A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.
Falkovich R, Danielson EW, Perez de Arce K, Wamhoff EC, Strother J, Lapteva AP, Sheng M, Cottrell JR, Bathe M
Cell reports (2023) 425: 112430. 182 105 ICC; tested species: rat

Neurons undergo pathogenic metabolic reprogramming in models of familial ALS.
Riechers SP, Mojsilovic-Petrovic J, Belton TB, Chakrabarty RP, Garjani M, Medvedeva V, Dalton C, Wong YC, Chandel NS, Dienel G, Kalb RG, et al.
Molecular metabolism (2022) 60: 101468. 182 105 ICC; tested species: rat

Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 182 105 ICC; tested species: mouse

Gating and modulation of a hetero-octameric AMPA glutamate receptor.
Zhang D, Watson JF, Matthews PM, Cais O, Greger IH
Nature (2021) 5947863: 454-458. 182 105 ICC; tested species: rat

Differential Scaling of Synaptic Molecules within Functional Zones of an Excitatory Synapse during Homeostatic Plasticity.
Venkatesan S, Subramaniam S, Rajeev P, Chopra Y, Jose M, Nair D
eNeuro (2020) 72: . 182 105 ICC; tested species: rat

Age-related dysregulation of homeostatic control in neuronal microcircuits.
Radulescu CI, Doostdar N, Zabouri N, Melgosa-Ecenarro L, Wang X, Sadeh S, Pavlidi P, Airey J, Kopanitsa M, Clopath C, Barnes SJ, et al.
Nature neuroscience (2023) 2612: 2158-2170. 182 105 IHC; tested species: mouse

Cat. No.: 182 105

Amount: 50 µg

Price: $465.00

Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 182 105 ICC; tested species: mouse

Gating and modulation of a hetero-octameric AMPA glutamate receptor.
Zhang D, Watson JF, Matthews PM, Cais O, Greger IH
Nature (2021) 5947863: 454-458. 182 105 ICC; tested species: rat

ICC

IHC

IHC-P

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Deng YP et al., J. Chem. Neuroanat. (2007)
4 ) Steiner P et al., EMBO J. (2005)
5 ) Nagy GG et al., J. Neurosci. (2004)
6 ) Passafaro M et al., Nature (2003)
7 ) Iihara K et al., J. Neurosci. (2001)
8 ) Daw MI et al., Neuron (2000)
9 ) Osten P et al., Neuron (1998)
10 ) He Y et al., Exp. Neurol. (1998)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

GluA2 antibody - 182 105

References for GluA2 - 182 105