Tailor-made Antibodies
and Tools for Life Science

GluA1 antibody - 182 011 K.O.

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission
Mouse monoclonal purified IgG
Cat. No.: 182 011
Amount: 100 µg
Price: $435.00
Cat. No. 182 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 200 gallery  
Clone 160E5
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 895 to 907 from rat GluA1 (UniProt Id: P19490)
Reactivity Reacts with: human (P42261), rat (P19490), mouse (P23818).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 182-01P
Remarks

WB: This antibody is less sensitive than the rabbit polyclonal antibody
(cat. no. 182 003).

Data sheet 182_011.pdf

References for GluA1 - 182 011

Human DDIT4L intron retention contributes to cognitive impairment and amyloid plaque formation.
Li KC, Shi HX, Li Z, You P, Pan J, Cai YC, Li JW, Ma XF, Zhang S, Diao L, Cai B, et al.
Cell discovery (2025) 111: 12. 182 011 WB, IHC; tested species: mouse
Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 182 011 WB, IHC; tested species: mouse
CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Zhu ZA, Li YY, Xu J, Xue H, Feng X, Zhu YC, Xiong ZQ
Cell reports (2023) 4210: 113202. 182 011 WB; tested species: mouse
PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus.
Lee DS, Kim TH, Park H, Kim JE
Scientific reports (2023) 131: 13927. 182 011 WB; tested species: mouse
AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats.
Kim JE, Lee DS, Park H, Kim TH, Kang TC
Biomedicines (2021) 98: . 182 011 WB, IP; tested species: rat
ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 182 011 WB; tested species: mouse
Chronic mild corticosterone exposure during adolescence enhances behaviors and upregulates neuroplasticity-related proteins in rat hippocampus.
Li J, Li Y, Sun Y, Wang H, Liu X, Zhao Y, Wang H, Su Y, Si T
Progress in neuro-psychopharmacology & biological psychiatry (2019) 89: 400-411. 182 011 WB; tested species: rat
Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 182 011 WB; tested species: mouse
Hippocampal Memory Recovery After Acute Stress: A Behavioral, Morphological and Molecular Study.
Aguayo FI, Tejos-Bravo M, Díaz-Véliz G, Pacheco A, García-Rojo G, Corrales W, Olave FA, Aliaga E, Ulloa JL, Avalos AM, Román-Albasini L, et al.
Frontiers in molecular neuroscience (2018) 11: 283. 182 011 WB; tested species: rat
Heterogeneity of Cell Surface Glutamate and GABA Receptor Expression in Shank and CNTN4 Autism Mouse Models.
Heise C, Preuss JM, Schroeder JC, Battaglia CR, Kolibius J, Schmid R, Kreutz MR, Kas MJH, Burbach JPH, Boeckers TM
Frontiers in molecular neuroscience (2018) 11: 212. 182 011 WB; tested species: mouse
STIM2 regulates AMPA receptor trafficking and plasticity at hippocampal synapses.
Yap KA, Shetty MS, Garcia-Alvarez G, Lu B, Alagappan D, Oh-Hora M, Sajikumar S, Fivaz M
Neurobiology of learning and memory (2017) 138: 54-61. 182 011 WB
Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats.
Pacheco A, Aguayo FI, Aliaga E, Muñoz M, García-Rojo G, Olave FA, Parra-Fiedler NA, García-Pérez A, Tejos-Bravo M, Rojas PS, Parra CS, et al.
Frontiers in molecular neuroscience (2017) 10: 244. 182 011 WB; tested species: rat
Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 182 011 WB; tested species: rat
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 182 011 WB, ICC
Synaptic function of nicastrin in hippocampal neurons.
Lee SH, Sharma M, Südhof TC, Shen J
Proceedings of the National Academy of Sciences of the United States of America (2014) 11124: 8973-8. 182 011 WB; tested species: mouse
Cat. No.: 182 011
Amount: 100 µg
Price: $435.00