Myobrevin (Vamp5) antibody - 176 011

Myobrevin or Vamp5 is a VAMP isoform expressed preferentially in skeletal muscle and heart

Mouse monoclonal purified IgG

Cat. No.: 176 011

Amount: 100 µg

Price: $420.00

Cat. No. 176 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: not recommended IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	162B2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 70 from mouse Myobrevin (UniProt Id: Q9Z2P8)
Reactivity	Reacts with: mouse (Q9Z2P8). No signal: rat. Other species not tested yet.
Matching control protein/peptide	176-0P
Data sheet	Datasheet 176_011

Cat. No.: 176 011

Amount: 100 µg

Price: $420.00

ICC

IHC-P (FFPE)

Background

Myobrevin, also known as VAMP 5 belongs to the family of vesicle-associated membrane proteins and has a theoretical molecular weight of 11.4 kDa. Like other VAMP isoforms it is composed of an N-terminal cytoplasmic region and a C-terminal transmembrane domain.
Vamp 5 is preferentially expressed in skeletal muscle and heart tissue and is upregulated during the differentiation of C2C12 cells into myotubes.

1 ) Brinkman JF et al., Mol. Vis. (2005)
2 ) Zeng Q et al., J. Biol. Chem. (2003)
3 ) Zeng Q et al., Mol. Biol. Cell (1998)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)

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