Synaptic Systems - Neurexin3

Cat. No.: 175 303

Amount: 50 µg

Price: $380.00

Processing of the synaptic cell adhesion molecule neurexin-3beta by Alzheimer disease alpha- and gamma-secretases.
Bot N, Schweizer C, Ben Halima S, Fraering PC
The Journal of biological chemistry (2011) 2864: 2762-73. 175 303 WB

WB

Background

α- and β-neurexins are single pass transmembrane proteins with a short cytoplasmic C-terminus and a long extracellular N-terminal part. In α-neurexins the extracellular sequence is substantially longer than in β-neurexins. Alternative splicing of the N-terminal part even confers more complexity to this protein family suggesting distinct binding partners for the extracellular regions. In contrast, the C-termini are highly conserved in the different isoforms and splice-variants and they share overlapping cytosolic binding partners.
Neurexins are receptor like molecules that form heterologous cell contacts with post-synaptic cell surface proteins at synaptic connections (e.g. β-neurexins with neuroligins). They also serve as receptors for the black widow toxin α-latrotoxin which induces neurotransmitter release.

1 ) Comoletti D et al., Structure (2007)
2 ) Craig AM et al., Curr. Opin. Neurobiol. (2007)
3 ) Chih B et al., Neuron (2006)
4 ) Lisé MF et al., Cell. Mol. Life Sci. (2006)
5 ) Paraoanu LE et al., Neurosci. Lett. (2006)
6 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)
7 ) Comoletti D et al., J. Biol. Chem. (2003)
8 ) Dean C et al., Nat. Neurosci. (2003)
9 ) Tabuchi K et al., Genomics (2002)
10 ) Tobaben S et al., J. Biol. Chem. (2002)

Protocols

WB

Cat. No. 175 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1298 to 1494 from rat Neurexin3 (UniProt Id: Q07310)
Reactivity	Reacts with: rat (Q07310), mouse (Q6P9K9). Other species not tested yet.
Specificity	Specific for neurexin 3. The epitope is present in α- and β-neurexin 3.
Data sheet	Datasheet 175_303

Neurexin3 antibody - 175 303

References for Neurexin3 - 175 303