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Home > Products > 174 203

Doc2a/b antibody - 174 203

Doc2 a/b are binding partners of Munc 13-1
Rabbit polyclonal purified antibody
Cat. No.: 174 203
Amount: 50 µg
Price: $375.00
Cat. No. 174 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 403 from rat Doc2a (UniProt Id: P70611)
Reactivity Reacts with: rat (P70611, P70610), mouse (Q7TNF0, P70169).
No signal: zebrafish.
Other species not tested yet.
Specificity Detects preferentially Doc 2a with some cross-reactivity to Doc 2b.
Data sheet 174_203.pdf

References for Doc2a/b - 174 203

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 174 203 WB
Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 174 203 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
Cat. No.: 174 203
Amount: 50 µg
Price: $375.00
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 174 203 WB
Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 174 203 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat
WB
ICC
IHC
Background

The Doc 2 protein family comprises the three isoforms Doc 2a, b and c. All three proteins are composed of a short N-terminal Munc 13 binding region followed by two C-terminal calcium binding domains (C2A and C2B). Doc 2a and b are predominantly expressed in brain and colocalize with small secretory vesicles whereas Doc 2c is mainly expressed in the heart.
The exact mechanism how the Doc proteins are involved in Ca2+ induced exocytosis has yet to be determined.