Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 174 103

Doc2b antibody - 174 103 K.O.

Doc2b is a binding partner of Munc 13-1
Rabbit polyclonal purified antibody
Cat. No.: 174 103
Amount: 50 µg
Price: $375.00
Cat. No. 174 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 38 to 119 from rat Doc2b (UniProt Id: P70610)
Reactivity Reacts with: rat (P70610), mouse (P70169).
Other species not tested yet.
Specificity Specific for Doc 2b, no cross-reactivity to Doc 2a and Doc 2c. K.O. validated PubMed: 32347796
Data sheet 174_103.pdf

References for Doc2b - 174 103

Disease-linked mutations in Munc18-1 deplete synaptic Doc2.
Guiberson NGL, Black LS, Haller JE, Brukner A, Abramov D, Ahmad S, Xie YX, Sharma M, Burré J
Brain : a journal of neurology (2024) : . 174 103 WB; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 174 103 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 174 103 WB; tested species: rat
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 174 103 ICC; tested species: rat
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions.
Khan MM, Regehr WG
eLife (2020) 9: . 174 103 IHC; KO verified; tested species: mouse
Cat. No.: 174 103
Amount: 50 µg
Price: $375.00
Disease-linked mutations in Munc18-1 deplete synaptic Doc2.
Guiberson NGL, Black LS, Haller JE, Brukner A, Abramov D, Ahmad S, Xie YX, Sharma M, Burré J
Brain : a journal of neurology (2024) : . 174 103 WB; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 174 103 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 174 103 WB; tested species: rat
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 174 103 ICC; tested species: rat
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions.
Khan MM, Regehr WG
eLife (2020) 9: . 174 103 IHC; KO verified; tested species: mouse
WB
Background

The Doc 2 protein family comprises the three isoforms Doc 2a, b and c. All three proteins are composed of a short N-terminal Munc 13 binding region followed by two C-terminal calcium binding domains (C2A and C2B). Doc 2a and b are predominantly expressed in brain and colocalize with small secretory vesicles whereas Doc 2c is mainly expressed in the heart.
The exact mechanism how the Doc proteins are involved in Ca2+ induced exocytosis has yet to be determined.