Tailor-made Antibodies
and Tools for Life Science

GFAP antibody - 173 211BT K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Mouse monoclonal purified IgG
Cat. No.: 173 211BT
Amount: 100 µg
Price: $470.00
Cat. No. 173 211BT 100 µg purified IgG, lyophilized, labeled with Biotin.
For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes (see remarks)
Label biotin
Clone 186C6
Subtype IgG1 (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms. K.O. validated
Matching control protein/peptide 173-0P
Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 173 011 as capture antibody.

Data sheet 173_211bt.pdf

References for GFAP - 173 211BT

O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies.
Permanne B, Sand A, Ousson S, Nény M, Hantson J, Schubert R, Wiessner C, Quattropani A, Beher D
ACS chemical neuroscience (2022) : . 173 211BT ELISA; tested species: mouse
A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates.
Ganau M, Bosco A, Palma A, Corvaglia S, Parisse P, Fruk L, Beltrami AP, Cesselli D, Casalis L, Scoles G
Nanomedicine : nanotechnology, biology, and medicine (2015) 112: 293-300. 173 211BT ELISA
Cat. No.: 173 211BT
Amount: 100 µg
Price: $470.00