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Home > Products > 173 011AT488

GFAP antibody - 173 011AT488 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Mouse monoclonal purified IgG
Cat. No.: 173 011AT488
Amount: 100 µg
Price: $465.00
Cat. No. 173 011AT488 100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet gallery  
IHC-P: not tested yet
Label ATTO 488
Clone 134B1
Subtype IgG2a (κ light chain)
Immunogen Full-length recombinant human GFAP (UniProt Id: P14136)
Epitop Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1 (alpha) K.O. validated
Matching control protein/peptide 173-0P
Data sheet 173_011at488.pdf
Cat. No.: 173 011AT488
Amount: 100 µg
Price: $465.00
ICC
ICC
IHC
IHC
IHC
IHC
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.
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