Tailor-made Antibodies
and Tools for Life Science

GFAP antibody - 173 009 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Chicken monoclonal recombinant IgY
Cat. No.: 173 009
Amount: 50 µg
Price: $420.00
Cat. No. 173 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
Clone Ch134B1
Subtype IgY (λ light chain)
Immunogen Full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 173-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 134B1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit antibody.

Data sheet 173_009.pdf
Cat. No.: 173 009
Amount: 50 µg
Price: $420.00