Synaptic Systems

Cat. No.: 173 006

Amount: 50 µg

Price: $390.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 173 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery DNA-PAINT: 1 : 500 iDISCO: 1 : 400
Immunogen	full-length recombinant human GFAP (UniProt Id: P14136)
Reactivity	Reacts with: human (P14136), rat (P47819), mouse (P03995). Other species not tested yet.
Specificity	Specific for GFAP, detects all isoforms. K.O. validated
Matching control protein/peptide	173-0P
Remarks	WB: Cat. nos. 173 002 or 173 011 are recommended.
Data sheet	173_006.pdf

References for GFAP - 173 006

WB (1)
ICC (1)
IHC (9)
IHC-P (2)
DNA-PAINT (1)

Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex.
Celine G, Thomas M
Cellular and molecular life sciences : CMLS (2025) 821: 102. 173 006 WB, IHC; tested species: mouse

A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.
Lyu Z, Park J, Kim KM, Jin HJ, Wu H, Rajadas J, Kim DH, Steinberg GK, Lee W
Nature biomedical engineering (2021) 58: 847-863. 173 006 ICC; tested species: human

Brain-gut photobiomodulation restores cognitive alterations in chronically stressed mice through the regulation of Sirt1 and neuroinflammation.
Sancho-Balsells A, Borràs-Pernas S, Flotta F, Chen W, Del Toro D, Rodríguez MJ, Alberch J, Blivet G, Touchon J, Xifró X, Giralt A, et al.
Journal of affective disorders (2024) : . 173 006 IHC; tested species: mouse

Induced Remodelling of Astrocytes In Vitro and In Vivo by Manipulation of Astrocytic RhoA Activity.
Domingos C, Müller FE, Passlick S, Wachten D, Ponimaskin E, Schwarz MK, Schoch S, Zeug A, Henneberger C
Cells (2023) 122: . 173 006 IHC; tested species: mouse

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 173 006 IHC; tested species: mouse

Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 173 006 IHC; tested species: mouse

Dopamine-induced calcium signaling in olfactory bulb astrocytes.
Fischer T, Scheffler P, Lohr C
Scientific reports (2020) 101: 631. 173 006 IHC; tested species: mouse

Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain.
Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, Farkas E, et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X19900097. 173 006 IHC; tested species: mouse

Amyloid β induces interneuron-specific changes in the hippocampus of APPNL-F mice.
Sos KE, Mayer MI, Takács VT, Major A, Bardóczi Z, Beres BM, Szeles T, Saito T, Saido TC, Mody I, Freund TF, et al.
PloS one (2020) 155: e0233700. 173 006 IHC; tested species: mouse

Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 173 006 IHC; tested species: mouse

Correction of dysregulated lipid metabolism normalizes gene expression in oligodendrocytes and prolongs lifespan in female poly-GA C9orf72 mice.
Rezaei A, Kocsis-Jutka V, Gunes ZI, Zeng Q, Kislinger G, Bauernschmitt F, Isilgan HB, Parisi LR, Kaya T, Franzenburg S, Koppenbrink J, et al.
Nature communications (2025) 161: 3442. 173 006 IHC-P; tested species: mouse

Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 173 006 IHC-P; tested species: mouse

Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 173 006 DNA-PAINT; tested species: rat

Cat. No.: 173 006

Amount: 50 µg

Price: $390.00

References
WB (1)
ICC (1)
IHC (9)
IHC-P (2)
DNA-PAINT (1)

Dopamine-induced calcium signaling in olfactory bulb astrocytes.
Fischer T, Scheffler P, Lohr C
Scientific reports (2020) 101: 631. 173 006 IHC; tested species: mouse

Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 173 006 DNA-PAINT; tested species: rat

ICC

IHC

IHC-P

Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.

1 ) Triolo D et al., J. Cell. Sci. (2006)
2 ) Shiihara T et al., J. Neurol. Sci. (2004)
3 ) Eng LF et al., Neurochem. Res. (2000)
4 ) Dyer CA et al., J. Neurosci. Res. (2000)
5 ) Valentino KL et al., Brain Res. (1983)
6 ) Palfreyman JW et al., J. Neurol. Sci. (1979)
7 ) Jacque CM et al., J. Neurol. Sci. (1978)

Protocols

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

GFAP antibody - 173 006 K.O.

References for GFAP - 173 006