Synaptic Systems - Vimentin

Cat. No.: 172 002

Amount: 200 µl

Price: $360.00

Cat. No. 172 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery EM: yes
Immunogen	Recombinant protein corresponding to AA 1 to 466 from mouse Vimentin (UniProt Id: P20152)
Reactivity	Reacts with: human (P08670), rat (P31000), mouse (P20152), monkey. No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 27419376
Data sheet	172_002.pdf

Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified

A Simple DMSO-Based Method for Cryopreservation of Primary Hippocampal and Cortical Neurons.
Ishizuka Y, Bramham CR
Journal of neuroscience methods (2019) : 108578. 172 002 WB; tested species: rat

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 172 002 WB

The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified

Cat. No.: 172 002

Amount: 200 µl

Price: $360.00

ICC

IHC

IHC-P

Background

Protocols

Vimentin antibody - 172 002 K.O.