Synaptic Systems - Vimentin

Cat. No.: 172 002

Amount: 200 µl

Price: $355.00

Cat. No. 172 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 1 to 466 from mouse Vimentin (UniProt Id: P20152)
Reactivity	Reacts with: human (P08670), rat (P31000), mouse (P20152), monkey. No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 27419376
Data sheet	172_002.pdf

References for Vimentin - 172 002

Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified

A Simple DMSO-Based Method for Cryopreservation of Primary Hippocampal and Cortical Neurons.
Ishizuka Y, Bramham CR
Journal of neuroscience methods (2019) : 108578. 172 002 WB; tested species: rat

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 172 002 WB

The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified

Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment.
Sugita S, Yamato M, Hatabu T, Kataoka Y
Scientific reports (2021) 111: 9668. 172 002 IHC; tested species: mouse

Tanycytes and a differential fatty acid metabolism in the hypothalamus.
Hofmann K, Lamberz C, Piotrowitz K, Offermann N, But D, Scheller A, Al-Amoudi A, Kuerschner L
Glia (2017) 652: 231-249. 172 002 IHC; tested species: mouse

KCa3.1 channels modulate the processing of noxious chemical stimuli in mice.
Lu R, Flauaus C, Kennel L, Petersen J, Drees O, Kallenborn-Gerhardt W, Ruth P, Lukowski R, Schmidtko A
Neuropharmacology (2017) 125: 386-395. 172 002 IHC; tested species: mouse

NDRG2 as a marker protein for brain astrocytes.
Flügge G, Araya-Callis C, Garea-Rodriguez E, Stadelmann-Nessler C, Fuchs E
Cell and tissue research (2014) 3571: 31-41. 172 002 IHC; tested species: marmoset

Age-related brain pathology in Octodon degu: blood vessel, white matter and Alzheimer-like pathology.
van Groen T, Kadish I, Popović N, Popović M, Caballero-Bleda M, Baño-Otálora B, Vivanco P, Rol MÁ, Madrid JA
Neurobiology of aging (2011) 329: 1651-61. 172 002 IHC

Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 172 002 IHC

Cat. No.: 172 002

Amount: 200 µl

Price: $355.00

ICC

IHC

IHC-P

Background

Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.

1 ) Styers ML et al., J. Cell. Sci. (2006)
2 ) Esue O et al., J. Biol. Chem. (2006)
3 ) Miyaguchi K et al., J. Struct. Biol. (1997)
4 ) Chiu FC et al., J. Neurochem. (1981)
5 ) Browning ET et al., J. Cell Biol. (1981)
6 ) Gard DL et al., Cell (1980)

Protocols

ICC

IHC

IHC-P

Other products for this target

Vimentin antibody - 172 002 K.O.

References for Vimentin - 172 002