Neurofilament M antibody - 171 241

Neurofilaments are major neuronal intermediate filaments

Mouse monoclonal purified IgG

Cat. No.: 171 241

Amount: 100 µg

Price: $415.00

Cat. No. 171 241	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 2000 up to 1 : 5000 gallery DNA-PAINT: 1 : 500 Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Clone	103H5A1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 761 to 846 from rat Neurofilament M (UniProt Id: P12839)
Reactivity	Reacts with: rat (P12839), mouse (P08553). Other species not tested yet.
Data sheet	171_241.pdf

References for Neurofilament M - 171 241

Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 171 241 DNA-PAINT; tested species: rat

Cat. No.: 171 241

Amount: 100 µg

Price: $415.00

Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 171 241 DNA-PAINT; tested species: rat

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

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6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

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