Cat. No. 171 211
|
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
|
Applications
|
WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 1000 (see remarks) gallery
Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
|
Clone
|
309F11
|
Subtype
|
IgG2b (κ light chain)
|
Immunogen
|
Recombinant protein corresponding to AA 763 to 848 from mouse Neurofilament M (UniProt Id: P08553)
|
Reactivity
|
Reacts with: mouse (P08553). No signal: rat (P12839). Other species not tested yet.
|
Remarks
|
IHC-P: Cat. no. 171 221 is more sensitive and recommended for this application.
|
Data sheet
|
171_211.pdf
|