Synaptic Systems - Neurofilament M

Cat. No.: 171 204

Amount: 100 µl

Price: $365.00

Cat. No. 171 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 763 to 848 from mouse Neurofilament M (UniProt Id: P08553)
Reactivity	Reacts with: rat (P12839), mouse (P08553). Other species not tested yet.
Data sheet	171_204.pdf

References for Neurofilament M - 171 204

Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC-derived neurons.
Dinamarca MC, Colombo L, Tousiaki NE, Müller M, Pecho-Vrieseling E
Frontiers in molecular biosciences (2022) 9: 916019. 171 204 ICC; tested species: human

Peptide backbone modifications of amyloid β (1-40) impact fibrillation behavior and neuronal toxicity.
Schwarze B, Korn A, Höfling C, Zeitschel U, Krueger M, Roßner S, Huster D
Scientific reports (2021) 111: 23767. 171 204 ICC; tested species: mouse

Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.
Potratz M, Zaeck LM, Weigel C, Klein A, Freuling CM, Müller T, Finke S
Acta neuropathologica communications (2020) 81: 199. 171 204 IHC; tested species: mouse

Autophagy is essential for oligodendrocyte differentiation, survival, and proper myelination.
Bankston AN, Forston MD, Howard RM, Andres KR, Smith AE, Ohri SS, Bates ML, Bunge MB, Whittemore SR
Glia (2019) : . 171 204 IHC; tested species: mouse

Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.
Mages B, Aleithe S, Altmann S, Blietz A, Nitzsche B, Barthel H, Horn AKE, Hobusch C, Härtig W, Krueger M, Michalski D, et al.
Frontiers in cellular neuroscience (2018) 12: 161. 171 204 IHC; tested species: human,mouse

Cat. No.: 171 204

Amount: 100 µl

Price: $365.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
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5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

Other products for this target

Neurofilament M antibody - 171 204

References for Neurofilament M - 171 204